Document Detail


ADP ribosyl cyclase activity of a novel bone marrow stromal cell surface molecule, BST-1.
MedLine Citation:
PMID:  7805847     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human BST-1, a bone marrow stromal cell surface molecule, is a GPI-anchored protein that facilitates the growth of pre-B cells. The deduced amino acid sequences of human and mouse BST-1 show around 30% homology with those of CD38 and Aplysia ADP ribosyl cyclase. Therefore, like CD38, BST-1 might possess ADP ribosyl cyclase activity. Here, we report the establishment of a stable transformant CHO cell line, which secretes truncated human soluble BST-1, and show that purified soluble BST-1 displays both ADP ribosyl cyclase and cADPR hydrolase activities.
Authors:
Y Hirata; N Kimura; K Sato; Y Ohsugi; S Takasawa; H Okamoto; J Ishikawa; T Kaisho; K Ishihara; T Hirano
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  356     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1994 Dec 
Date Detail:
Created Date:  1995-01-27     Completed Date:  1995-01-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  244-8     Citation Subset:  IM    
Affiliation:
Fuji Gotemba Research Labs. Chugai Pharmaceutical Co. Ltd., Shizuoka, Japan.
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MeSH Terms
Descriptor/Qualifier:
ADP-ribosyl Cyclase
Animals
Antigens, CD*
Antigens, CD38
Antigens, Differentiation / biosynthesis,  chemistry,  metabolism*
Base Sequence
Bone Marrow / metabolism
Bone Marrow Cells*
CHO Cells
Cations, Divalent / pharmacology
Cricetinae
DNA Primers
Humans
Hydrogen-Ion Concentration
Kinetics
Membrane Glycoproteins / biosynthesis,  chemistry,  metabolism*
Molecular Sequence Data
N-Glycosyl Hydrolases / biosynthesis,  chemistry,  metabolism*
NAD / metabolism
Polymerase Chain Reaction
Recombinant Proteins / biosynthesis,  chemistry,  metabolism
Stromal Cells / metabolism*
Transfection
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, Differentiation; 0/CD157 antigen; 0/Cations, Divalent; 0/DNA Primers; 0/Membrane Glycoproteins; 0/Recombinant Proteins; 53-84-9/NAD; EC 3.2.2.-/N-Glycosyl Hydrolases; EC 3.2.2.5/ADP-ribosyl Cyclase; EC 3.2.2.5/Antigens, CD38; EC 3.2.2.5/CD38 protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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