Document Detail

ADAMTS13 activity is decreased in a septic porcine model. Significance for glomerular thrombus deposition.
MedLine Citation:
PMID:  21057705     Owner:  NLM     Status:  MEDLINE    
During sepsis, the balance between abundantly secreted von Willebrand factor (VWF) and the activity of its size regulating protease ADAMTS13 is assumed to be involved in coagulation abnormalities. We aimed to establish a porcine model with haemorrhagic shock with consecutive sepsis and hypothesised that a decreased ADAMTS13-activity as well as an altered VWF multimer pattern is associated with renal failure. Animals (n=21) were subjected to haemorrhagic shock. After volume replacement, intraperitoneal Escherichia coli sepsis was induced. Blood samples were drawn at baseline, after haemorrhage and sepsis induction. Directly postmortem we examined renal tissue by JONES-silver, CD61, VWF and fibrin staining for characterisation of thrombi. Renal failure was analysed by scoring PAS-stained sections for acute tubular damage. Glomerular microthrombi were observed in six of 21 septic animals. Porcine ADAMTS13 activity declined significantly during sepsis, accompanied by a drop-off in platelet count. At 12 hours after sepsis induction, ADAMTS13 activity was significantly diminished compared to sham controls, and an elevated acute tubular damage score was associated with an increased proportion of high-molecular-weight VWF multimers. Compared to baseline the proportion of high-molecular-weight VWF multimers increased significantly in septic animals. Similar to human sepsis, diminished ADAMTS13 activity was observed in a septic porcine model associated with a shift to rather thrombogenic VWF multimers and deposition of microthrombi. Therefore, this porcine model seems to be appropriate for performing functional and therapeutic studies in sepsis-associated ADAMTS13 deficiency.
Clemens L Bockmeyer; Phillipp A Reuken; Tim P Simon; Ulrich Budde; Wolfgang Lösche; Michael Bauer; Ingvild Birschmann; Jan U Becker; Gernot Marx; Ralf A Claus
Related Documents :
3871585 - Botrocetin- and polybrene-induced platelet aggregation in platelet-type von willebrand ...
10702705 - Acquired bernard-soulier syndrome: a case with necrotizing vasculitis and thrombosis.
3875375 - Eccentric localization of von willebrand factor in an internal structure of platelet al...
1939645 - Von willebrand factor binding to platelet gpib initiates signals for platelet activation.
17506225 - Influence of antidepressants on hemostasis.
15149885 - Activation of paf receptor by oxidised ldl in human monocytes stimulates chemokine rele...
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-11-05
Journal Detail:
Title:  Thrombosis and haemostasis     Volume:  105     ISSN:  0340-6245     ISO Abbreviation:  Thromb. Haemost.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-04     Completed Date:  2011-05-18     Revised Date:  2011-05-23    
Medline Journal Info:
Nlm Unique ID:  7608063     Medline TA:  Thromb Haemost     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  145-53     Citation Subset:  IM    
Department of Anaesthesiology and Intensive Care, Jena University Hospital, Jena, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
ADAM Proteins / metabolism*
Disease Models, Animal*
Escherichia coli
Protein Multimerization
Renal Insufficiency / etiology
Sepsis / complications*
Shock, Hemorrhagic / complications*
Thrombosis / etiology*
von Willebrand Factor / metabolism
Reg. No./Substance:
0/von Willebrand Factor; EC 3.4.24.-/ADAM Proteins; EC 3.4.24.-/ADAMTS13 protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Microparticle-associated tissue factor activity and venous thrombosis in multiple myeloma.
Next Document:  Quantitative expression analysis and study of the novel human kallikrein-related peptidase 14 gene (...