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ADAMTS: Novel Proteases Expressed by Activated Mast Cells.
MedLine Citation:
PMID:  23154421     Owner:  NLM     Status:  Publisher    
Abstract Here we show that mast cells (MCs) express metalloproteases of the A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) family, and that ADAMTS expression is influenced by MC activation. Co-culture of MCs with live Gram-positive bacteria caused a profound induction of ADAMTS-9 and -6, as well as downregulated expression of ADAMTS-5. Similar patterns were also seen after MC activation with calcium ionophore and by IgE receptor crosslinking. Moreover, ADAMTS-5, -6 and -9 were all induced by activation of terminally differentiated murine peritoneal MCs and in a human MC line. ADAMTS-9 upregulation in response to IgE receptor crosslinking was strongly dependent on Gö6976-sensitive PKC and partly dependent on NFAT and NF-κB, respectively. The expression of ADAMTS-5, -6 and -9 was closely linked to MC maturation, as shown by their strong induction during maturation of bone marrow precursor cells into mature MC phenotype. ADAMTS family members have been shown to possess aggrecanase activity. Accordingly, MCs were shown to express aggrecanase activity. Finally, ADAMTS-5 protein was detected in MCs by immunocytochemistry. Taken together, the present study reveals ADAMTS expression by MCs and that MC activation regulates the expression of these proteases, thus implicating the ADAMTS family of proteases in MC function.
Gianni García-Faroldi; Elin Rönnberg; Adolfo Orro; Gabriela Calounova; Bengt Guss; Anders Lundequist; Gunnar Pejler
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-16
Journal Detail:
Title:  Biological chemistry     Volume:  -     ISSN:  1437-4315     ISO Abbreviation:  Biol. Chem.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9700112     Medline TA:  Biol Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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