Document Detail


ADAMTS-1 and ADAMTS-4 levels are elevated in thoracic aortic aneurysms and dissections.
MedLine Citation:
PMID:  23245439     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) is a recently identified family of extracellular metalloproteinases that has been shown to participate in tissue destruction. We hypothesized that ADAMTS-1 and ADAMTS-4 expression is increased in aortic tissues from patients with thoracic aortic aneurysms and dissections.
METHODS: We examined ADAMTS-1 and ADAMTS-4 expression in human descending thoracic aortic aneurysms (n = 14), chronic descending thoracic aortic dissections (n = 16), and descending thoracic aortas from age-matched control organ donors (n = 12). In these tissues, we also evaluated the degradation of versican, a proteoglycan substrate of ADAMTS-1 and ADAMTS-4. In cultured macrophages, we examined whether ADAMTS-4 functions in macrophage infiltration by using a transwell assay.
RESULTS: ADAMTS-1 and ADAMTS-4 protein and mRNA expression was significantly higher in thoracic aortic aneurysm and dissection tissues than in control aortic tissues. Double immunofluorescence staining showed the expression of ADAMTS-1 and ADAMTS-4 in smooth muscle cells and macrophages. Consistent with the upregulation of ADAMTS-1 and ADAMTS-4 in thoracic aortic aneurysm and dissection tissues, versican was degraded significantly more in these tissues than in control aortic tissues. In cultured macrophages, transforming growth factor-β increased ADAMTS-4 protein levels and induced macrophage invasion, and the knockdown of ADAMTS-4 reduced cell invasion.
CONCLUSIONS: Increased expression of ADAMTS proteins may promote thoracic aortic aneurysm progression by degrading versican and facilitating macrophage invasion.
Authors:
Pingping Ren; Lin Zhang; Gaiping Xu; Laura C Palmero; Paul T Albini; Joseph S Coselli; Ying H Shen; Scott A LeMaire
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-12-13
Journal Detail:
Title:  The Annals of thoracic surgery     Volume:  95     ISSN:  1552-6259     ISO Abbreviation:  Ann. Thorac. Surg.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-22     Completed Date:  2013-03-26     Revised Date:  2014-02-04    
Medline Journal Info:
Nlm Unique ID:  15030100R     Medline TA:  Ann Thorac Surg     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  570-7     Citation Subset:  AIM; IM    
Copyright Information:
Copyright © 2013 The Society of Thoracic Surgeons. Published by Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
ADAM Proteins / blood*
Aneurysm, Dissecting / blood*
Aortic Aneurysm, Thoracic / blood*
Female
Humans
Male
Middle Aged
Procollagen N-Endopeptidase / blood*
Grant Support
ID/Acronym/Agency:
5R01HL085341/HL/NHLBI NIH HHS; P50 HL083794/HL/NHLBI NIH HHS; P50 HL083794/HL/NHLBI NIH HHS; R01 HL085341/HL/NHLBI NIH HHS; UL1 RR024148/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
EC 3.4.24.-/ADAM Proteins; EC 3.4.24.-/ADAMTS1 protein, human; EC 3.4.24.-/aggrecanase-1; EC 3.4.24.14/Procollagen N-Endopeptidase
Comments/Corrections
Comment In:
Ann Thorac Surg. 2013 Feb;95(2):577-8   [PMID:  23336870 ]
Ann Thorac Surg. 2013 Sep;96(3):1124-5   [PMID:  23992724 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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