Document Detail


ADAM 12 may be used to reduce the false positive rate of first trimester combined screening for Down syndrome.
MedLine Citation:
PMID:  20013872     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: ADAM12 has been shown to be an efficient maternal serum marker for Down syndrome (DS) in the first trimester; but recent studies, using a second generation assay, have not confirmed these findings. We examined the efficiency of a second generation assay for ADAM12. MATERIALS AND METHODS: ADAM12 concentrations were determined in 28 first trimester DS and 503 control pregnancies using a novel Research Delfia ADAM12 kit. Log10MoM distributions of ADAM12 and correlations with other markers were established. Population performance of screening was estimated by Monte Carlo simulation. RESULTS: ADAM12 was significantly reduced in the first trimester in DS pregnancies with a log10MoM of -0.1621 (equivalent to 0.68 MoM) (p < 0.001). The reduction decreased with advancing gestational age. ADAM12 used with PAPP-A + hCG beta + NT (CUB screening) increased the detection rate (DR) from 86% to 89% for a false positive rate (FPR) of 5%. When used for a fixed DR of 90%, the addition of ADAM12 resulted in a 25% reduction of the FPR. CONCLUSION: ADAM12 is a moderately effective DS marker. It is not a cost-effective addition to CUB screening, but may be used to reduce the FPR in selected high-risk cases.
Authors:
Michael Christiansen; Kasper Pihl; Paula L Hedley; Anne-Cathrine Gjerris; Pia ? Lind; Severin Olesen Larsen; Lone Krebs; Torben Larsen
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Prenatal diagnosis     Volume:  30     ISSN:  1097-0223     ISO Abbreviation:  Prenat. Diagn.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-01     Completed Date:  2010-04-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8106540     Medline TA:  Prenat Diagn     Country:  England    
Other Details:
Languages:  eng     Pagination:  110-4     Citation Subset:  IM    
Copyright Information:
(c) 2009 John Wiley & Sons, Ltd.
Affiliation:
Department of Clinical Biochemistry and Immunology, Statens Serum Institut, Copenhagen, Denmark. mic@ssi.dk
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MeSH Terms
Descriptor/Qualifier:
ADAM Proteins / blood*,  diagnostic use
Adult
Biological Markers
Case-Control Studies
Down Syndrome / blood*,  diagnosis*
False Positive Reactions
Female
Humans
Mass Screening
Membrane Proteins / blood*,  diagnostic use
Pregnancy
Pregnancy Trimester, First
Prenatal Care
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Membrane Proteins; EC 3.4.24.-/ADAM 12 protein; EC 3.4.24.-/ADAM Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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