Document Detail


ACTH response to corticotropin releasing hormone in Cushing's disease before and after ketoconazole: in vivo and in vitro studies.
MedLine Citation:
PMID:  2832292     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ketoconazole has been used as a palliative treatment of Cushing's syndrome, due to its ability to lower cortisol production. We evaluated the effects of ovine Corticotropin Releasing Hormone (oCRH) 100 micrograms i.v. on ACTH and cortisol levels in 6 patients with Cushing's disease before and after treatment with ketoconazole 600 mg/day. Both hormones increased after oCRH. During ketoconazole, cortisol was lowered to normal levels and its response to oCRH was impaired. After treatment, basal ACTH showed variable changes while the response to oCRH was markedly enhanced compared to that before ketoconazole. In vitro: In a continuous perfusion system of isolated anterior pituitary cells from rats or human anterior pituitary adenoma, producing ACTH, ketoconazole 10(-5)-10(-6) M showed no inhibitory effects on both basal or lisine-vasopressin and oCRH stimulated ACTH secretion. Our findings confirm the inhibitory action of ketoconazole on basal and stimulated cortisol secretion. No inhibition of ACTH levels was observed both in vivo and in vitro.
Authors:
M Boscaro; N Sonino; A Rampazzo; A M Menegus; F Mantero
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Hormone and metabolic research. Supplement series     Volume:  16     ISSN:  0170-5903     ISO Abbreviation:  Horm. Metab. Res. Suppl.     Publication Date:  1987  
Date Detail:
Created Date:  1988-05-10     Completed Date:  1988-05-10     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0330417     Medline TA:  Horm Metab Res Suppl     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  60-2     Citation Subset:  IM    
Affiliation:
Institute of Semeiotica Medica, University of Padua, Italy.
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MeSH Terms
Descriptor/Qualifier:
Adenoma / secretion
Adrenocorticotropic Hormone / secretion*
Adult
Animals
Corticotropin-Releasing Hormone / diagnostic use*
Cushing Syndrome / drug therapy,  physiopathology*
Female
Humans
Hydrocortisone / secretion
Ketoconazole / pharmacology*,  therapeutic use
Kinetics
Male
Middle Aged
Pituitary Gland, Anterior / drug effects,  secretion
Pituitary Neoplasms / secretion
Rats
Rats, Inbred Strains
Chemical
Reg. No./Substance:
50-23-7/Hydrocortisone; 65277-42-1/Ketoconazole; 9002-60-2/Adrenocorticotropic Hormone; 9015-71-8/Corticotropin-Releasing Hormone

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