Document Detail


ACE2 is required for daughter cell-specific G1 delay in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  12937340     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Saccharomyces cerevisiae cells reproduce by budding to yield a mother cell and a smaller daughter cell. Although both mother and daughter begin G1 simultaneously, the mother cell progresses through G1 more rapidly. Daughter cell G1 delay has long been thought to be due to a requirement for attaining a certain critical cell size before passing the commitment point in the cell cycle known as START. We present an alternative model in which the daughter cell-specific Ace2 transcription factor delays G1 in daughter cells. Deletion of ACE2 produces daughter cells that proceed through G1 at the same rate as mother cells, whereas a mutant Ace2 protein that is not restricted to daughter cells delays G1 equally in both mothers and daughters. The differential in G1 length between mothers and daughters requires the Cln3 G1 cyclin, and CLN3-GFP reporter expression is reduced in daughters in an ACE2-dependent manner. Specific daughter delay elements in the CLN3 promoter are required for normal daughter G1 delay, and these elements bind to an unidentified 127-kDa protein. This DNA-binding activity is enhanced by deletion of ACE2. These results support a model in which daughter cell G1 delay is determined not by cell size but by an intrinsic property of the daughter cell generated by asymmetric cell division.
Authors:
Tracy L Laabs; David D Markwardt; Matthew G Slattery; Laura L Newcomb; David J Stillman; Warren Heideman
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.     Date:  2003-08-22
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  100     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-09-03     Completed Date:  2003-10-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  10275-80     Citation Subset:  IM    
Affiliation:
School of Pharmacy, University of Wisconsin, Madison, WI 53705, USA.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Cell Cycle
Cell Size
Cyclins / genetics,  physiology
DNA-Binding Proteins / physiology*
G1 Phase / physiology*
Molecular Sequence Data
Promoter Regions, Genetic
Saccharomyces cerevisiae / physiology*
Saccharomyces cerevisiae Proteins / genetics,  physiology*
Transcription Factors / physiology*
Grant Support
ID/Acronym/Agency:
GM48624/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/ACE2 protein, S cerevisiae; 0/CLN3 protein, S cerevisiae; 0/Cyclins; 0/DNA-Binding Proteins; 0/Saccharomyces cerevisiae Proteins; 0/Transcription Factors
Comments/Corrections

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