Document Detail


An AC electrokinetics facilitated biosensor cassette for rapid pathogen identification.
MedLine Citation:
PMID:  23626988     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To develop a portable point-of-care system based on biosensors for common infectious diseases such as urinary tract infection, the sensing process needs to be implemented within an enclosed fluidic system. On chip sample preparation of clinical samples remains a significant obstacle to achieving robust sensor performance. Herein AC electrokinetics is applied in an electrochemical biosensor cassette to enhance molecular convection and hybridization efficiency through electrokinetics induced fluid motion and Joule heating induced temperature elevation. Using E. coli as an exemplary pathogen, we determined the optimal electrokinetic parameters for detecting bacterial 16S rRNA in the biosensor cassette based on the current output, signal-to-noise ratio, and limit of detection. In addition, a panel of six probe sets targeting common uropathogenic bacteria was demonstrated. The optimized parameters were also validated using patient-derived clinical urine samples. The effectiveness of electrokinetics for on chip sample preparation will facilitate the implementation of point-of-care diagnosis of urinary tract infection in the future.
Authors:
Mengxing Ouyang; Ruchika Mohan; Yi Lu; Tingting Liu; Kathleen E Mach; Mandy L Y Sin; Mason McComb; Janhvi Joshi; Vincent Gau; Pak Kin Wong; Joseph C Liao
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Analyst     Volume:  138     ISSN:  1364-5528     ISO Abbreviation:  Analyst     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-06-04     Completed Date:  2013-12-03     Revised Date:  2014-07-13    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  England    
Other Details:
Languages:  eng     Pagination:  3660-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacteria / genetics,  isolation & purification*
Biosensing Techniques / methods*
DNA, Bacterial / analysis,  chemistry
Electrochemistry
Humans
Nucleic Acid Hybridization
Oligonucleotide Probes / chemistry
Point-of-Care Systems
Thermometry
Time Factors
Urine / microbiology
Grant Support
ID/Acronym/Agency:
DP2 OD007161/OD/NIH HHS; DP2 OD007161/OD/NIH HHS; R44 AI 088756/AI/NIAID NIH HHS; U01 AI082457/AI/NIAID NIH HHS; U01 AI082457/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/Oligonucleotide Probes
Comments/Corrections

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