Document Detail

An AC electrokinetic technique for collection and concentration of particles and cells on patterned electrodes.
MedLine Citation:
PMID:  15982074     Owner:  NLM     Status:  PubMed-not-MEDLINE    
We report an electrohydrodynamic effect arising from the application of alternating electric fields to patterned electrode surfaces. The AC fields were applied to dilute suspensions of latex microspheres enclosed between a patterned silicon wafer and an ITO-coated glass slide in a small chamber. The latex particles became collected in the center of the conductive "corrals" on the silicon wafer acting as bottom electrode. The particle collection efficiency and speed depended only on the frequency and strength of the field and were independent of the material properties of the particles or the electrodes. The leading effect in the particle collection process is AC electrohydrodynamics. We discuss how the electrohydrodynamic flows emerge from the spatially nonuniform field and interpret the experimental results by means of electrostatic and hydrodynamic simulations. The technique allows three-dimensional microfluidic pumping and transport by the use of two-dimensional patterns. We demonstrate on-chip collection of latex particles, yeast cells, and microbes.
Ketan H Bhatt; Sonia Grego; Orlin D Velev
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Langmuir : the ACS journal of surfaces and colloids     Volume:  21     ISSN:  0743-7463     ISO Abbreviation:  Langmuir     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-06-28     Completed Date:  2006-08-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882736     Medline TA:  Langmuir     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6603-12     Citation Subset:  -    
Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina 27695-7905, USA.
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