Document Detail


ABRF ESRG 2005 study: identification of seven modified amino acids by Edman sequencing.
MedLine Citation:
PMID:  17122064     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Identification of modified amino acids can be a challenging part for Edman degradation sequence analysis, largely because they are not included among the commonly used phenylthiohydantion amino acid standards. Yet many can have unique retention times and can be assigned by an experienced researcher or through the use of a guide showing their typical chromatography characteristics. The Edman Sequencing Research Group (ESRG) 2005 study is a continuation of the 2004 study, in which the participating laboratories were provided a synthetic peptide and asked to identify the modified amino acids present in the sequence. The study sample provided an opportunity to sequence a peptide containing a variety of modified amino acids and note their retention times relative to the common amino acids. It also allowed the ESRG to compile the chromatographic properties and intensities from multiple instruments and tabulate an average elution position for these modified amino acids on commonly used instruments. Participating laboratories were given 2000 pmoles of a synthetic peptide, 18 amino acids long, containing the following modified amino acids: dimethyl- and trimethyl-lysine, 3-methyl-histidine, N-carbamyl-lysine, cystine, N-methyl-alanine, and isoaspartic acid. The modified amino acids were interspersed with standard amino acids to help in the assessment of initial and repetitive yields. In addition to filling in an assignment sheet, which included retention times and peak areas, participants were asked to provide specific details about the parameters used for the sequencing run. References for some of the modified amino acid elution characteristics were provided and the participants had the option of viewing a list of the modified amino acids present in the peptide at the ESRG Web site. The ABRF ESRG 2005 sample is the seventeenth in a series of studies designed to aid laboratories in evaluating their abilities to obtain and interpret amino acid sequence data.
Authors:
D Brune; N D Denslow; R Kobayashi; W S Lane; J W Leone; B J Madden; J M Neveu; J Pohl
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomolecular techniques : JBT     Volume:  17     ISSN:  1524-0215     ISO Abbreviation:  J Biomol Tech     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-11-23     Completed Date:  2007-10-19     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  100888641     Medline TA:  J Biomol Tech     Country:  United States    
Other Details:
Languages:  eng     Pagination:  308-26     Citation Subset:  IM    
Affiliation:
Arizona State University, Tempe, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Amino Acids / analysis*,  chemistry*
Molecular Sequence Data
Organophosphorus Compounds
Peptides / chemistry
Phenylthiohydantoin / chemistry
Sequence Analysis, Protein*
Chemical
Reg. No./Substance:
0/2-(4-isothiocyanatophenoxy)-1,3,2-dioxaphosphinene 2-oxide; 0/Amino Acids; 0/Organophosphorus Compounds; 0/Peptides; 2010-15-3/Phenylthiohydantoin
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