| ABO Genotyping by Capillary Electrophoresis. | |
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MedLine Citation:
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PMID: 22976095 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Single-strand conformation polymorphism by capillary electrophoresis (SSCP-CE) has been developed to detect single nucleotide mutations. This method is used to identify the ABO alleles A(1), A(1v), B, O(1), O(1v), and O(2) in this chapter. Four amplicons (112, 121, 123, and 160 bp) labeled with fluorescence are separately amplified by polymerase chain reaction from exons 6 and 7 of ABO gene. These four fragments are combined into a single tube for SSCP-CE analysis using native gel to identify their single nucleotide polymorphism. This method can fast screen ABO genotypes from unknown samples and will be valuable in clinical transfusion or forensic applications. |
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Authors:
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James Chun-I Lee; Hsing-Mei Hsieh; Hsiao-Feng Teng; Li-Chin Tsai |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 919 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-09-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 113-20 Citation Subset: IM |
Affiliation:
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Department of Forensic Medicine, National Taiwan University, Taipei, Taiwan ROC. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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