Document Detail


The ABC's of Group IV cytosolic phospholipase A2.
MedLine Citation:
PMID:  15164769     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The three known human Group IV phospholipase A2 (PLA2) paralogs, Group IVA, IVB and IVC, were overexpressed in Sf9 insect cells using the baculovirus expression system. An endogenous, calcium-independent PLA2 activity was identified in the insect cell lysates, which can be inhibited by bromoenol lactone (BEL). The Group IV PLA2 enzymes were characterized in overexpressing insect cell lysates in the presence of BEL, enabling their differentiation from the endogenous PLA2 activity. Group IVC PLA2 was found to have significant lysophospholipase activity, while Group IVB PLA2 did not. Of the three paralogs, only the Group IVA PLA2 shows enhanced activity in the presence of PIP2, which enables its differential detection in cell homogenates. RT-PCR was used to demonstrate the presence of all three enzymes in human U937 and human WISH cells, while only Group IVA and Group IVB PLA2 were detected in murine P388D1 cells and human astrocytes at the mRNA level.
Authors:
Karin Killermann Lucas; Edward A Dennis
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1636     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-05-28     Completed Date:  2004-06-29     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  213-8     Citation Subset:  IM    
Affiliation:
Department of Chemistry and Biochemistry, School of Medicine, University of California at San Diego, La Jolla, CA 92093-0601, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Cytosol / enzymology*
DNA Primers
DNA, Complementary
Humans
Phospholipases A / genetics,  metabolism*
Phospholipases A2
Recombinant Proteins / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Spodoptera
Grant Support
ID/Acronym/Agency:
GM 20501/GM/NIGMS NIH HHS; GM 64611/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Complementary; 0/Recombinant Proteins; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2

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