Document Detail


ABI4 activates DGAT1 expression in Arabidopsis seedlings during nitrogen deficiency.
MedLine Citation:
PMID:  21515696     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Triacylglycerol (TAG) is the major seed storage lipid and is important for biofuel and other renewable chemical uses. Acyl-coenzyme A:diacylglycerol acyltransferase1 (DGAT1) is the rate-limiting enzyme in the TAG biosynthesis pathway, but the mechanism of its regulation is unknown. Here, we show that TAG accumulation in Arabidopsis (Arabidopsis thaliana) seedlings increased significantly during nitrogen deprivation (0.1 mm nitrogen) with concomitant induction of genes involved in TAG biosynthesis and accumulation, such as DGAT1 and OLEOSIN1. Nitrogen-deficient seedlings were used to determine the key factors contributing to ectopic TAG accumulation in vegetative tissues. Under low-nitrogen conditions, the phytohormone abscisic acid plays a crucial role in promoting TAG accumulation in Arabidopsis seedlings. Yeast one-hybrid and electrophoretic mobility shift assays demonstrated that ABSCISIC ACID INSENSITIVE4 (ABI4), an important transcriptional factor in the abscisic acid signaling pathway, bound directly to the CE1-like elements (CACCG) present in DGAT1 promoters. Genetic studies also revealed that TAG accumulation and DGAT1 expression were reduced in the abi4 mutant. Taken together, our results indicate that abscisic acid signaling is part of the regulatory machinery governing TAG ectopic accumulation and that ABI4 is essential for the activation of DGAT1 in Arabidopsis seedlings during nitrogen deficiency.
Authors:
Yang Yang; Xiangchun Yu; Lianfen Song; Chengcai An
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-04-22
Journal Detail:
Title:  Plant physiology     Volume:  156     ISSN:  1532-2548     ISO Abbreviation:  Plant Physiol.     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-06-02     Completed Date:  2011-09-26     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  873-83     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China.
Data Bank Information
Bank Name/Acc. No.:
RefSeq/NM_112304;  NM_113376;  NM_115011;  NM_117778;  NM_118646;  NM_120486;  NM_121367;  NM_125910;  NM_129185;  NM_129318;  NM_129580;  NM_172503
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MeSH Terms
Descriptor/Qualifier:
Abscisic Acid / pharmacology
Arabidopsis / drug effects,  genetics*
Arabidopsis Proteins / genetics*,  metabolism
Base Sequence
Diacylglycerol O-Acyltransferase / genetics*,  metabolism
Gene Expression Regulation, Plant*
Genes, Plant / genetics
Molecular Sequence Data
Mutation / genetics
Nitrogen / deficiency*,  pharmacology
Plant Oils / metabolism
Plants, Genetically Modified
Promoter Regions, Genetic / genetics
Protein Binding / drug effects
Seedling / drug effects,  genetics*
Tobacco / drug effects,  genetics
Transcription Factors / metabolism*
Transcription, Genetic / drug effects
Triglycerides / biosynthesis
Chemical
Reg. No./Substance:
0/ABI4 protein, Arabidopsis; 0/Arabidopsis Proteins; 0/Plant Oils; 0/Transcription Factors; 0/Triglycerides; 21293-29-8/Abscisic Acid; 7727-37-9/Nitrogen; EC 2.3.1.20/Diacylglycerol O-Acyltransferase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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