Document Detail


AAV-mediated overexpression of human α7 integrin leads to histological and functional improvement in dystrophic mice.
MedLine Citation:
PMID:  23319059     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Duchenne muscular dystrophy (DMD) is a severe muscle disease caused by mutations in the DMD gene, with loss of its gene product, dystrophin. Dystrophin helps link integral membrane proteins to the actin cytoskeleton and stabilizes the sarcolemma during muscle activity. We investigated an alternative therapeutic approach to dystrophin replacement by overexpressing human α7 integrin (ITGA7) using adeno-associated virus (AAV) delivery. ITGA7 is a laminin receptor in skeletal and cardiac muscle that links the extracellular matrix (ECM) to the actin skeleton. It is modestly upregulated in DMD muscle and has been proposed to be an important modifier of dystrophic symptoms. We delivered rAAV8.MCK.ITGA7 to the lower limb of mdx mice through isolated limb perfusion (ILP) of the femoral artery. We demonstrated ~50% of fibers in the tibialis anterior (TA) and extensor digitorum longus (EDL) overexpressing α7 integrin at the sarcolemma following AAV gene transfer. The increase in ITGA7 in skeletal muscle significantly protected against loss of force following eccentric contraction-induced injury compared with untreated (contralateral) muscles while specific force following tetanic contraction was unchanged. Reversal of additional dystrophic features included reduced Evans blue dye (EBD) uptake and increased muscle fiber diameter. Taken together, this data shows that rAAV8.MCK.ITGA7 gene transfer stabilizes the sarcolemma potentially preserving mdx muscle from further damage. This therapeutic approach demonstrates promise as a viable treatment for DMD with further implications for other forms of muscular dystrophy.
Authors:
Kristin N Heller; Chrystal L Montgomery; Paul Ml Janssen; K Reed Clark; Jerry R Mendell; Louise R Rodino-Klapac
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-01-15
Journal Detail:
Title:  Molecular therapy : the journal of the American Society of Gene Therapy     Volume:  21     ISSN:  1525-0024     ISO Abbreviation:  Mol. Ther.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-03-01     Completed Date:  2013-10-22     Revised Date:  2014-06-30    
Medline Journal Info:
Nlm Unique ID:  100890581     Medline TA:  Mol Ther     Country:  United States    
Other Details:
Languages:  eng     Pagination:  520-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD / genetics*,  metabolism
Dependovirus / genetics*
Disease Models, Animal
Dystrophin / genetics,  metabolism
Extracellular Matrix / metabolism
Gene Transfer Techniques
Genetic Therapy / methods
Genetic Vectors*
Humans
Integrin alpha Chains / genetics*,  metabolism
Mice
Mice, Inbred mdx
Muscle, Skeletal / metabolism,  physiopathology
Muscular Dystrophy, Duchenne / genetics,  physiopathology,  therapy*
Mutation
Sarcolemma / genetics
Up-Regulation
Grant Support
ID/Acronym/Agency:
1U54HD066409/HD/NICHD NIH HHS; U54 HD066409/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Dystrophin; 0/Integrin alpha Chains; 0/integrin alpha7
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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