Document Detail


AAL toxins, fumonisins (biology and chemistry) and host-specificity concepts.
MedLine Citation:
PMID:  1513374     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The AAL toxins and the fumonisins (FB1 and FB2) are structurally related and produced respectively by Alternaria alternata f.sp. lycopersici and Fusarium moniliforme. AAL toxin is characterized as a host-specific toxin, toxic to tomato, whereas fumonisin B1 causes equine leukoencephalomalacia. FB1 and FB2 were biologically active in susceptible tomato tissue (Earlypak-7) and animal tissue culture (rat hepatoma H4TG and dog kidney MDCK). Conversely, AAL toxin was also active in the rat and dog tissue culture cells. Both fungi produce toxin/s in culture that causes death in rats; these toxins are other than AAL and fumonisin. The peracetylated derivatives of AAL and FB1 are biologically inactive in both the tomato bioassay and the animal tissue culture systems. Acetylation of the amine renders AAL inactive. The hydrolysis product of AAL (phentolamine) is toxic to the susceptible tomato line whereas the phentolamine of fumonisin is not. AAL and FB1 can be analyzed by Continuous Flow Fast Atom Bombardment (CFFAB) and Ionspray Mass Spectrometry (ISM), both sensitive to the picomole range. The N-acetyl of the TFA hydrolysis product of AAL and FB1 is determined by comparing the fragment ions at m/z 86 and 140 for FB1 and 72 and 126 for AAL.
Authors:
C J Mirocha; D G Gilchrist; W T Shier; H K Abbas; Y Wen; R F Vesonder
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Mycopathologia     Volume:  117     ISSN:  0301-486X     ISO Abbreviation:  Mycopathologia     Publication Date:  1992 Feb 
Date Detail:
Created Date:  1992-10-01     Completed Date:  1992-10-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7505689     Medline TA:  Mycopathologia     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  47-56     Citation Subset:  IM    
Affiliation:
Department of Plant Pathology, University of Minnesota, St. Paul 55108.
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MeSH Terms
Descriptor/Qualifier:
Alternaria*
Animals
Cell Line
Dogs
Encephalomalacia / chemically induced,  veterinary
Female
Fumonisins*
Fusarium*
Horse Diseases / chemically induced
Horses
Mass Spectrometry
Mice
Mycotoxins / biosynthesis,  chemistry,  toxicity*
Rats
Species Specificity
Spectrometry, Mass, Fast Atom Bombardment
Sphingosine*
Structure-Activity Relationship
Swine
Chemical
Reg. No./Substance:
0/Alternaria alternata pathotoxin TA; 0/Fumonisins; 0/Mycotoxins; 116355-83-0/fumonisin B1; 116355-84-1/fumonisin B2; 123-78-4/Sphingosine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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