Document Detail

A2B5 cells from human glioblastoma have cancer stem cell properties.
MedLine Citation:
PMID:  19243384     Owner:  NLM     Status:  MEDLINE    
Glioblastomas, like other cancers, harbor small cell populations with the capability of sustaining tumor formation. These cells are referred to as cancer stem cells. We isolated cells expressing the surface marker A2B5 from three human glioblastomas (GBM) and showed that after grafting into nude mice, they generated dense and highly infiltrative tumors. Then, we extensively studied A2B5(+) cells isolated from 11 human GBM. These cells display neurosphere-like, self-renewal, asymmetrical cell division properties and have multipotency capability. Stereotactic xenografts of dissociated A2B5(+)-derived secondary spheres revealed that as few as 1000 cells produced a tumor. Moreover, flow cytometry characterization of A2B5(+)-derived spheres revealed three distinct populations of cells: A2B5(+)/CD133(+), A2B5(+)/CD133(-) and A2B5(-)/CD133(-), with striking proportion differences among GBM. Both A2B5(+)/CD133(+) and A2B5(+)/CD133(-) cell fractions displayed a high proliferative index, the potential to generate spheres and produced tumors in nude mice. Finally, we generated two green fluorescent protein-cell lines that display--after serum induction--distinct proliferative and migratory properties, and differ in their CD133 level of expression. Taken together, our results suggest that transformed A2B5(+) cells are crucial for the initiation and maintenance of GBM, although CD133 expression is more involved in determining the tumor's behavior.
Aurélie Tchoghandjian; Nathalie Baeza; Carole Colin; Myriam Cayre; Philippe Metellus; Christophe Beclin; L'Houcine Ouafik; Dominique Figarella-Branger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-02-20
Journal Detail:
Title:  Brain pathology (Zurich, Switzerland)     Volume:  20     ISSN:  1750-3639     ISO Abbreviation:  Brain Pathol.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-05-04     Completed Date:  2010-07-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9216781     Medline TA:  Brain Pathol     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  211-21     Citation Subset:  IM    
Université de la Méditerranée Aix-Marseille II, Faculté de Médecine la Timone, INSERM UMR911-CRO2, Angiogenèse, Invasivité et Microenvironnement Tumoral, Marseille cedex 05, France.
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MeSH Terms
Antigens, CD / metabolism
Brain / pathology
Brain Neoplasms / pathology*
Cell Line, Tumor
Cell Movement
Cell Proliferation
Cell Separation
Cell Transplantation
Fluorescent Antibody Technique
Glioblastoma / pathology*
Glycoproteins / metabolism
Green Fluorescent Proteins / biosynthesis,  genetics
Mice, Nude
Neoplastic Stem Cells / physiology*
Peptides / metabolism
Reg. No./Substance:
0/AC133 antigen; 0/Antigens, CD; 0/Glycoproteins; 0/Peptides; 147336-22-9/Green Fluorescent Proteins

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