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A(2B) adenosine receptor blockade inhibits growth of prostate cancer cells.
MedLine Citation:
PMID:  23315335     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The role of the A(2B) adenosine receptor (AR) in prostate cell death and growth was studied. The A(2B) AR gene expression quantified by real-time quantitative RT-PCR and Western blot analysis was the highest among four AR subtypes (A(1), A(2A), A(2B), and A(3)) in all three commonly used prostate cancer cell lines, PC-3, DU145, and LNCaP. We explored the function of the A(2B) AR using PC-3 cells as a model. The A(2B) AR was visualized in PC-3 cells by laser confocal microscopy. The nonselective A(2B) AR agonist NECA and the selective A(2B) AR agonist BAY60-6583, but not the A(2A) AR agonist CGS21680, concentration-dependently induced adenosine 3',5'-cyclic monophosphate (cyclic AMP) accumulation. NECA diminished lactate dehydrogenase (LDH) release, TNF-α-induced increase of caspase-3 activity, and cycloheximide (CHX)-induced morphological changes typical of apoptosis in PC-3 cells, which were blocked by a selective A(2B) AR antagonist PSB603. NECA-induced proliferation of PC-3 cells was diminished by siRNA specific for the A(2B) AR. The selective A(2B) AR antagonist PSB603 was shown to inhibit cell growth in all three cell lines. Thus, A(2B) AR blockade inhibits growth of prostate cancer cells, suggesting selective A(2B) AR antagonists as potential novel therapeutics.
Authors:
Qiang Wei; Stefano Costanzi; Ramachandran Balasubramanian; Zhan-Guo Gao; Kenneth A Jacobson
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-15
Journal Detail:
Title:  Purinergic signalling     Volume:  -     ISSN:  1573-9546     ISO Abbreviation:  Purinergic Signal.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101250499     Medline TA:  Purinergic Signal     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Molecular Recognition Section, Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, 20892-0810, USA.
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