| 9p21 deletion in the diagnosis of malignant mesothelioma, using fluorescence in situ hybridization analysis. | |
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MedLine Citation:
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PMID: 20518890 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Homozygous deletion of 9p21, the locus harboring the p16 gene, has been reported as one of the most common genetic alterations in malignant mesotheliomas (MMs). Previous studies showed that this alteration might be useful for differentiating benign from malignant mesothelial tumors in cytology and surgical specimens. Although the diagnostic utility of 9p21 homozygous deletion by fluorescence in situ hybridization (FISH) analysis has been reported only recently, it has not been well demonstrated. The purpose of this study is to evaluate the diagnostic utility of 9p21 homozygous deletion assessed by FISH in mesothelial neoplasm and hyperplasia of Japanese patients using paraffin-embedded tissue. Simultaneously, p16 protein immunoexpression was explored as a potential diagnostic aid. FISH analysis demonstrated 9p21 deletion in 35 of 40 cases with MM (88%) (P < 0.001). In contrast, no cases of adenomatoid tumor, benign mesothelial multicystic tumor, reactive mesothelial hyperplasia or pleuritis showed 9p21 deletion (P < 0.005). 9p21 homozygous deletion correlated well with p16 protein expression in the tumor cells. Our study suggests that 9p21 homozygous deletion assessed by FISH on paraffin-embedded tissue may be very useful for differentiating MM from reactive mesothelial proliferation. |
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Authors:
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Maiko Takeda; Takahiko Kasai; Yasunori Enomoto; Masato Takano; Kouhei Morita; Eiji Kadota; Akitaka Nonomura |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Pathology international Volume: 60 ISSN: 1440-1827 ISO Abbreviation: Pathol. Int. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-06-03 Completed Date: 2010-09-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9431380 Medline TA: Pathol Int Country: Australia |
Other Details:
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Languages: eng Pagination: 395-9 Citation Subset: IM |
Affiliation:
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Department of Diagnostic Pathology, Nara Medical University School of Medicine, Nara, Japan. maikot@naramed-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chromosomes, Human, Pair 9* DNA, Neoplasm / analysis Epithelium / pathology Female Gene Deletion Gene Dosage Genes, p16* Heart Neoplasms / diagnosis*, genetics, metabolism Humans In Situ Hybridization, Fluorescence / methods* Mesothelioma / diagnosis*, genetics, metabolism Pericardium / metabolism, pathology Peritoneal Neoplasms / diagnosis*, genetics, metabolism Pleural Neoplasms / diagnosis*, genetics, metabolism Tumor Markers, Biological / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/DNA, Neoplasm; 0/Tumor Markers, Biological |
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