Document Detail


9-cis-retinoic acid selectively activates the cellular retinoic acid binding protein-II gene in human neuroblastoma cells.
MedLine Citation:
PMID:  7786028     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two families of nuclear retinoid receptors, retinoic acid receptor and retinoid X receptor (RAR and RXR respectively), and a family of cellular retinoic acid-binding proteins (CRABPI and II) participate in the retinoic acid (RA) signaling pathway. The presence and function of many of these receptors and cellular binding proteins have not been fully explored in RA-responsive human neuroblastoma cells. We have previously shown that RAR transcripts and protein are present in human neuroblastoma cells, and that all-trans RA induces the expression of the RAR beta mRNA. In this paper, we demonstrate that human neuroblastoma cells express mRNA for RXR alpha and beta. The mRNA for CRABPI is present in untreated human neuroblastoma cells, whereas the mRNA for CRABPII is induced in cells treated with either all-trans RA or 9-cis RA. Furthermore, 9-cis RA, a ligand that binds to both the RAR and the RXR families, selectively activates the CRABPII gene. In contrast, all-trans RA and 9-cis RA are equally effective in the induction of RAR beta transcript and inhibition of cell proliferation. Since both retinoids inhibit human neuroblastoma cell proliferation, it appears that induction of RAR beta rather than of CRABPII is more likely linked to the regulation of human neuroblastoma cell growth.
Authors:
L A Plum; M Clagett-Dame
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  319     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  1995 Jun 
Date Detail:
Created Date:  1995-07-17     Completed Date:  1995-07-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  457-63     Citation Subset:  IM    
Affiliation:
Interdepartmental Graduate Program in Nutritional Sciences, School of Pharmacy, University of Wisconsin-Madison 53706, USA.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Cell Division / drug effects
Gene Expression Regulation, Neoplastic
Humans
Molecular Sequence Data
Neuroblastoma / genetics,  metabolism*
Polymerase Chain Reaction
RNA, Messenger / biosynthesis
Receptors, Retinoic Acid / biosynthesis*,  genetics
Tretinoin / pharmacology*
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
DK-14881/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/Receptors, Retinoic Acid; 302-79-4/Tretinoin

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