| 9-cis and all-trans retinoic acid induce a similar phenotype in human teratocarcinoma cells. | |
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MedLine Citation:
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PMID: 8243889 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Prior work has shown that all-trans retinoic acid (t-RA) treatment of the human teratocarcinoma (TC) cell line NTERA-2 clone D1 (abbreviated NT2/D1) induces a neuronal phenotype and other cell lineages. This study sought to explore the potential of 9-cis retinoic acid (9-cis RA) as a differentiation-inducing agent of this multipotent cell. Findings reported here show that 9-cis RA induced a phenotype similar to t-RA treatment of NT2/D1 cells. This similarity extended to their effects on the nuclear receptors retinoic acid receptor-beta (RAR-beta) and retinoid X receptor-alpha (RXR-alpha). Both retinoids prominently augmented RAR-beta expression and transactivated a reporter plasmid containing putative RAR response elements (RAREs) with direct repeats separated by five nucleotides (DR5). Both retinoids had no appreciable effect on RXR-alpha expression and both minimally transactivated a reporter plasmid containing putative RXR response elements (RXREs) with direct repeats separated by one nucleotide (DR1). These studies suggest that 9-cis RA and t-RA activate common events during retinoid-mediated NT2/D1 differentiation. This hypothesis was supported by the finding that NT2/D1 cells rendered refractory to t-RA (NT2/D1-R1) were also resistant to 9-cis RA. To discover alterations that could confer retinoid-refractoriness, retinoid receptor expression was examined in NT2/D1-R1 cells. In contrast to NT2/D1, the NT2/D1-R1 cell was found to have reduced RXR-alpha expression at the level of total cellular RNA.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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J M Kurie; J Buck; T M Eppinger; D Moy; E Dmitrovsky |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Differentiation; research in biological diversity Volume: 54 ISSN: 0301-4681 ISO Abbreviation: Differentiation Publication Date: 1993 Sep |
Date Detail:
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Created Date: 1993-12-23 Completed Date: 1993-12-23 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0401650 Medline TA: Differentiation Country: GERMANY |
Other Details:
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Languages: eng Pagination: 123-9 Citation Subset: IM |
Affiliation:
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Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, NY 10021. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Differentiation
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drug effects,
genetics Drug Resistance / genetics Humans Neurons / drug effects Phenotype Receptors, Retinoic Acid / biosynthesis Stereoisomerism Teratocarcinoma / genetics* Tretinoin / chemistry, pharmacology* Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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1F32CA08940-02/CA/NCI NIH HHS; 1RO1-CA54494-02/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Retinoic Acid; 302-79-4/Tretinoin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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