Document Detail

An 8-week high-fat diet induces obesity and insulin resistance with small changes in the muscle transcriptome of C57BL/6J mice.
MedLine Citation:
PMID:  20588053     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Skeletal muscle is responsible for most of the insulin-stimulated glucose uptake and metabolism. Therefore, it plays an important role in the development of insulin resistance, one of the characteristics of the metabolic syndrome (MS). As the prevalence of the MS is increasing, there is an urgent need for more effective intervention strategies.
METHODS: C57BL/6J mice were fed an 8-week low-fat diet (10 kcal%; LFD) or high-fat diet (45 kcal%; HFD). Microarray analysis was performed by using two comparisons: (1) 8-week HFD transcriptome versus 8-week LFD transcriptome and (2) transcriptome of mice sacrificed at the start of the intervention versus 8-week LFD transcriptome and 8-week HFD transcriptome, respectively.
RESULTS: Although an 8-week HFD induced obesity and impaired insulin sensitivity, HFD-responsive changes in the muscle transcriptome were relatively small (<1.3-fold). In fact, 8-weeks of aging induced more pronounced changes than an HFD. One comparison revealed the transcriptional downregulation of the mito- gen-activated protein kinase cascade, whereas both comparisons showed the upregulation of fatty acid oxidation, demonstrating that the two comparison strategies are confirmative as well as complementary.
CONCLUSION: We suggest using complementary analysis strategies in the genome-wide search for gene expression changes induced by mild interventions, such as an HFD.
Janneke de Wilde; Egbert Smit; Ronny Mohren; Mark V Boekschoten; Philip de Groot; Sjoerd A A van den Berg; Silvia Bijland; Peter J Voshol; Ko Willems van Dijk; Nicole W J de Wit; Annelies Bunschoten; Gert Schaart; Martijn F M Hulshof; Edwin C M Mariman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-28
Journal Detail:
Title:  Journal of nutrigenetics and nutrigenomics     Volume:  2     ISSN:  1661-6499     ISO Abbreviation:  J Nutrigenet Nutrigenomics     Publication Date:  2009  
Date Detail:
Created Date:  2010-08-19     Completed Date:  2010-12-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101299758     Medline TA:  J Nutrigenet Nutrigenomics     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  280-91     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 S. Karger AG, Basel.
Nutrigenomics Consortium, Top Institute Food and Nutrition, Wageningen University, Wageningen, The Netherlands.
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MeSH Terms
Adiponectin / blood
Adipose Tissue / anatomy & histology
Body Composition
Diet, Fat-Restricted
Dietary Fats / adverse effects*
Gene Expression Profiling*
Gene Expression Regulation
Glucose / metabolism
Insulin Resistance*
Mice, Inbred C57BL
Muscle, Skeletal / drug effects,  physiology*,  physiopathology
Obesity / chemically induced*
Oligonucleotide Array Sequence Analysis
Organ Specificity
RNA / genetics,  isolation & purification
Transcription, Genetic / drug effects
Weight Gain
Reg. No./Substance:
0/Adiponectin; 0/Dietary Fats; 50-99-7/Glucose; 63231-63-0/RNA

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