| 8-Hydroxyguanine levels and repair capacity during mouse embryonic stem cell differentiation. | |
| | |
MedLine Citation:
|
PMID: 21291352 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Abstracts To evaluate the defence capacities of embryonic stem (ES) cells against gene impairment, this study measured the levels of 8-hydroxyguanine (8-OH-Gua), a well-known marker of oxidative stress in DNA, and its repair capacity during differentiation. Undifferentiated ES cells (EB3) were cultured without leukaemia inhibitory factor (LIF) for 0, 4 and 7 days and are referred to as ES-D0, ES-D4 and ES-D7, respectively. These three cell lines were treated with 300 ?M hydrogen peroxide (H(2)O(2)) for 48 and 72 h. After treatment, the amounts of 8-OH-Gua in the cells were determined by the high-performance liquid chromatography (HPLC)-electrochemical detector (ECD) method. The levels of 8-OH-Gua in ES-D7 treated with H(2)O(2) were higher than those in ES-D0 and ES-D4, suggesting that the DNA in the undifferentiated cells was protected against gene impairment, as compared to that in the differentiated cells. To examine the repair capacity for 8-OH-Gua, this study analysed the expression of 8-OH-Gua repair-associated genes, 8-oxoguanine DNA glycosylase 1 (OGG1), MutY homolog (MUTYH) and Mut T homolog 1 (MTH1), in ES-D0, ES-D4 and ES-D7. The mRNA levels of MUTYH and MTH1 showed no significant change, whereas OGG1 mRNA was significantly decreased in ES-D7 treated with H(2)O(2). Moreover, it was observed that ES-D7 treated with H(2)O(2) readily underwent apoptosis, in comparison to its undifferentiated counterparts, ES-D0 and ES-D4. Taken together, ES cells are more resistant to DNA oxidative stresses than differentiated cells. |
| | |
Authors:
|
Ayumi Kuboyama; Shoichi Tanaka; Kazuaki Kawai; Hiroshi Kasai; Haruki Morii; Kazuyoshi Tamae; Tamiji Nakashima; Takeshi Hirano |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-2-4 |
Journal Detail:
|
Title: Free radical research Volume: - ISSN: 1029-2470 ISO Abbreviation: - Publication Date: 2011 Feb |
Date Detail:
|
Created Date: 2011-2-4 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9423872 Medline TA: Free Radic Res Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Chemical Processes and Environments, Faculty of Environmental Engineering, The University of Kitakyushu, Kitakyushu, Fukuoka, 808-0135, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effect of exercise on bone and articular cartilage in heterozygous manganese superoxide dismutase (S...
Next Document: Alrp, a survival factor that controls the apoptotic process of regenerating liver after partial hepa...