Document Detail

8-Bromo-cAMP mimics beta-adrenergic sensitization of GABA responses to ethanol in cerebellar Purkinje neurons in vivo.
MedLine Citation:
PMID:  8730238     Owner:  NLM     Status:  MEDLINE    
Previous studies in our laboratory indicated that electrophysiological responses of cerebellar Purkinje neurons to GABA were not routinely potentiated by ethanol (EtOH), and the potentiation was not large when it occurred. In the presence of beta-adrenergic agonists, such as isoproterenol, however, GABA inhibitions became sensitive to potentiation by EtOH in nearly every Purkinje neuron tested. beta-adrenergic receptor activation alone also modulates (potentiates) GABA responses on Purkinje neurons, and this has been reported to be mediated by a cAMP second messenger system. Herein, we report that the membrane-permeable cAMP analog, 8-bromoadenosine-3',5'-cyclic monophosphate (8-Br-cAMP), but not the membrane-impermeable cAMP, can also modulate GABA responses and that EtOH potentiates this facilitatory action of 8-Br-cAMP. These effects are not likely caused by adenosine receptor mechanisms, because this 8-bromoadenosine mediated modulation and sensitization was observed in the presence of systemic theophylline. These data suggest that the beta-adrenergic modulation and sensitization to EtOH of cerebellar Purkinje neuron GABA responses occur via a cAMP second messenger mechanism.
R K Freund; M R Palmer
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Alcoholism, clinical and experimental research     Volume:  20     ISSN:  0145-6008     ISO Abbreviation:  Alcohol. Clin. Exp. Res.     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1996-10-02     Completed Date:  1996-10-02     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7707242     Medline TA:  Alcohol Clin Exp Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  408-12     Citation Subset:  IM    
Department of Pharmacology, University of Colorado Health Sciences Center, Denver 80262, USA.
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MeSH Terms
8-Bromo Cyclic Adenosine Monophosphate / pharmacology*
Cerebellum / drug effects*
Dose-Response Relationship, Drug
Ethanol / pharmacology*
Purkinje Cells / drug effects*
Rats, Sprague-Dawley
Receptors, Adrenergic, beta / drug effects*
Second Messenger Systems / drug effects
gamma-Aminobutyric Acid / pharmacology*
Grant Support
Reg. No./Substance:
0/Receptors, Adrenergic, beta; 23583-48-4/8-Bromo Cyclic Adenosine Monophosphate; 56-12-2/gamma-Aminobutyric Acid; 64-17-5/Ethanol

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