| 8-Br-cyclic GMP given during reperfusion improves post-transplant lung edema and free radical injury. | |
| | |
MedLine Citation:
|
PMID: 10703694 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Substitution of the NO-pathway reduces ischemia/reperfusion injury following lung transplantation. 8-Br-cGMP is a membrane permeable analogue of cGMP, the second messenger of NO. In this study the effect of continuous administration of 8-Br-cGMP on early graft function was evaluated. METHODS: Unilateral left lung transplantation was performed in 10 weight-matched pigs (23-30 kg). Donor lungs were flushed with 1.51 cold (1 degree C) LPD solution and preserved for 20 hours. In Group I (n = 5), 8-Br-cGMP (0.2 mg/kg/h) was given continuously over the entire observation time starting 15 min before reperfusion. Group II served as control, no 8-Br-cGMP was administered. In both groups, 250 microg PGE1 was injected into the pulmonary artery (PA) before flush. One hour after reperfusion the recipients contralateral right PA and bronchus were ligated to assess isolated graft function only. Extravascular lung water index (EVLWI), pulmonary vascular resistance, mean PA pressure, mean systemic arterial pressure and gas exchange were assessed during a 5-hour observation period. Lipid peroxidation as indicator for free radical mediated injury and neutrophil migration to the allograft were measured at the end of the assessment. RESULTS: EVLWI was significantly reduced in animals treated with 8-Br-cGMP (overall difference P = 0.024) with a peak 2 hours after reperfusion (Group I, 8.2+/-0.3 mg/ml vs Group II, 10.1+/-0.6 mg/ml; P = 0.039). Also in Group I the free radical mediated tissue injury was significantly lower when compared to Group II (Group I, 61.8+/-12.3 pmol/g vs Group II, 120.7+/-7.2 pmol/g; P = 0.006). A tendency towards a reduced neutrophil migration after 8-Br-cGMP infusion was shown; however, the changes in comparison to the control animals were not statistically significant (Group I, 1.0+/-0.2 deltaOD/mg/min vs Group II, 1.7+/-0.3 deltaOD/mg/min; P = 0.13). Pulmonary- and systemic hemodynamics, and allograft gas exchange did not differ between groups. CONCLUSIONS: The results indicate that substitution of the NO pathway by administration of the second messenger cGMP at the time of reperfusion improves post-transplant lung allograft function. |
| | |
Authors:
|
P Sandera; S Hillinger; U Stammberger; G Schoedon; M Zalunardo; W Weder; R A Schmid |
Related Documents
:
|
14704474 - Cardiomyocyte mitochondrial katp channels participate in the antiarrhythmic and antiinf... 21879984 - Pharmacokinetics of levetiracetam after oral and intravenous administration of a single... 16716834 - Risperidone attenuates brain damage after focal cerebral ischemia in vivo. 2350874 - High dose versus low dose enteral allopurinol for prophylaxis in mesenteric ischemia. 1419604 - No effect of dose, hepatic function, or nutritional status on 5-fu clearance following ... 15123224 - One week treatment with salmeterol does not prevent early and late asthmatic responses ... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation Volume: 19 ISSN: 1053-2498 ISO Abbreviation: J. Heart Lung Transplant. Publication Date: 2000 Feb |
Date Detail:
|
Created Date: 2000-03-15 Completed Date: 2000-03-15 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 9102703 Medline TA: J Heart Lung Transplant Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 173-8 Citation Subset: IM |
Affiliation:
|
Department of Internal Medicine, University Hospital, Zürich, Switzerland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cyclic GMP / analogs & derivatives*, therapeutic use Cyclic GMP-Dependent Protein Kinases / antagonists & inhibitors* Extravascular Lung Water Free Radicals Lipid Peroxidation Lung Transplantation* Postoperative Period Pulmonary Edema / drug therapy* Random Allocation Reperfusion Injury / physiopathology, prevention & control* Swine Thiobarbituric Acid Reactive Substances Transplantation, Homologous |
| Chemical | |
Reg. No./Substance:
|
0/Free Radicals; 0/Thiobarbituric Acid Reactive Substances; 31356-94-2/8-bromocyclic GMP; 7665-99-8/Cyclic GMP; EC 2.7.11.12/Cyclic GMP-Dependent Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Intravascular ultrasound imaging after cardiac transplantation: advantage of multi-vessel imaging.
Next Document: Differential time scale of fluid and solute permeability following hypothermic lung preservation.