Document Detail


7beta-hydroxycholesterol induces apoptosis and regulates cyclooxygenase 2 in head and neck squamous cell carcinoma.
MedLine Citation:
PMID:  19289704     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To determine whether treatment with 7beta-hydroxycholesterol (7beta-HC) would trigger cell death in head and neck squamous cell carcinoma (HNSCC) cell lines in a dose-dependent fashion. DESIGN: In vitro study. SUBJECTS: The study included HNSCC cell lines SCC9, SCC25, CAL27, and FaDu. INTERVENTION: We treated HNSCC cell lines with increasing doses of 7beta-HC. Proliferation assays were performed to assess cell viability after treatment. Western blots were carried out to evaluate cyclooxygenase (COX)-1 and -2 expression levels. RESULTS: Using proliferation assays and immunocytochemical analysis, we detected significant growth inhibition via apoptosis in 4 different HNSCC cell lines after treatment with 7beta-HC (P < .001). The 50% inhibitory concentration levels were between 13.19 and 20.79 micromol/L after 72 hours. Western analysis indicated that COX-2, but not COX-1, levels were suppressed after treatment. CONCLUSIONS: Treatment with 7beta-HC resulted in suppression of HNSCC growth in vitro. Our data warrant further investigations for the potential use of 7beta-HC as a cytotoxic agent in head and neck cancer.
Authors:
Gregor Heiduschka; Boban M Erovic; Laurenz Vormittag; Christiane Skoda; Helga Martinek; Markus Brunner; Klaus Ehrenberger; Dietmar Thurnher
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Archives of otolaryngology--head & neck surgery     Volume:  135     ISSN:  1538-361X     ISO Abbreviation:  Arch. Otolaryngol. Head Neck Surg.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-17     Completed Date:  2009-04-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8603209     Medline TA:  Arch Otolaryngol Head Neck Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  261-7     Citation Subset:  AIM; IM    
Affiliation:
Department of Otorhinolaryngology-Head and Neck Surgery, Medical University of Vienna, Vienna, Austria.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects*
Blotting, Western
Carcinoma, Squamous Cell / drug therapy,  enzymology*,  pathology
Cell Count
Cell Line, Tumor
Cell Proliferation / drug effects
Cyclooxygenase 2 / drug effects,  metabolism*
Dose-Response Relationship, Drug
Flow Cytometry
Head and Neck Neoplasms / drug therapy,  enzymology*,  pathology
Humans
Hydroxycholesterols / pharmacology*
Immunohistochemistry
Chemical
Reg. No./Substance:
0/Hydroxycholesterols; 566-26-7/cholest-5-en-3 beta,7 alpha-diol; EC 1.14.99.1/Cyclooxygenase 2

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