Document Detail

7Li nuclear magnetic resonance study for the determination of Li+ properties in neuroblastoma SH-SY5Y cells.
MedLine Citation:
PMID:  9751202     Owner:  NLM     Status:  MEDLINE    
Lithium has been used clinically in the treatment of manic depression. However, its pharmacologic mode of action remains unclear. Characteristics of Li+ interactions in red blood cells (RBCs) have been identified. We investigated Li+ interactions on human neuroblastoma SH-SY5Y cells by developing a novel 7Li NMR method that provided a clear estimation of the intra- and extracellular amounts of Li+ in the presence of the shift reagent thulium-1,4,7,10-tetrazacyclododecane-N,N',N'',N'''-tetramethylene phosphonate (HTmDOTP4-). The first-order rate constants of Li+ influx and efflux for perfused, agarose-embedded SH-SY5Y cells in the presence of 3 mM HTmDOTP4- were 0.055 +/- 0.006 (n = 4) and -0.025 +/- 0.006 min(-1) (n = 3), respectively. Significant increases in the rate constants of Li+ influx and efflux in the presence of 0.05 mM veratridine indicated the presence of Na+ channel-mediated Li+ transport in SH-SY5Y cells. 7Li NMR relaxation measurements showed that Li+ is immobilized more in human neuroblastoma SH-SY5Y cells than in human RBCs.
J Nikolakopoulos; C Zachariah; D Mota de Freitas; E B Stubbs; R Ramasamy; M C Castro; C F Geraldes
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  71     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1998-10-14     Completed Date:  1998-10-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1676-84     Citation Subset:  IM    
Department of Chemistry, Loyola University Chicago, Illinois 60626, USA.
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MeSH Terms
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / pharmacology
Cell Survival / drug effects
Intracellular Fluid / chemistry,  metabolism
Ion Transport / drug effects
Lithium / chemistry*,  metabolism*,  pharmacology
Magnetic Resonance Spectroscopy
Neuroblastoma / chemistry*,  metabolism*,  pathology
Ouabain / pharmacology
Phloretin / pharmacology
Tumor Cells, Cultured
Veratridine / pharmacology
Grant Support
Reg. No./Substance:
0/Isotopes; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 60-82-2/Phloretin; 630-60-4/Ouabain; 71-62-5/Veratridine; 7439-93-2/Lithium

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