| A 796 bp PsPR10 gene promoter fragment increased root-specific expression of the GUS reporter gene under the abiotic stresses and signal molecules in tobacco. | |
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MedLine Citation:
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PMID: 20495947 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A 1681 bp PsPR10 promoter was isolated from Pinus strobus and a series of 5'-deletions were fused to the β-glucuronidase (GUS) reporter gene and introduced into tobacco. GUS activity in P796 (-796 to +69) construct transgenic plant roots was similar with that of P1681 and higher than those of the P513 (-513 to +69) and P323 (-323 to +69) transgenic plants. Moreover, the abiotic stresses of NaCl, PEG 6000 and mannitol, and salicylic acid (SA), abscisic acid (ABA) and jasmonic acid (JA) induced higher GUS activity in the roots of P796 transgenic tobacco. This study provides a potential inducible root-specific promoter for transgenic plants. |
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Authors:
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Xiangbin Xu; Sai Guo; Kai Chen; Hongmiao Song; Junjun Liu; Longbiao Guo; Qian Qian; Huizhong Wang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-22 |
Journal Detail:
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Title: Biotechnology letters Volume: 32 ISSN: 1573-6776 ISO Abbreviation: Biotechnol. Lett. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-17 Completed Date: 2011-01-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1533-9 Citation Subset: IM |
Affiliation:
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College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/HM036116 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Artificial Gene Fusion DNA, Plant / chemistry, genetics Glucuronidase / biosynthesis*, genetics Molecular Sequence Data Pinus / genetics Plant Roots / enzymology, genetics*, metabolism*, physiology Plants, Genetically Modified / enzymology, genetics, metabolism, physiology Promoter Regions, Genetic* Recombinant Proteins / biosynthesis, genetics Sequence Analysis, DNA Sequence Deletion Stress, Physiological* Tobacco / enzymology, genetics*, metabolism*, physiology |
| Chemical | |
Reg. No./Substance:
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0/DNA, Plant; 0/Recombinant Proteins; EC 3.2.1.31/Glucuronidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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