Document Detail


On the 710 nm fluorescence emitted by the diatom Phaeodactylum tricornutum at room temperature.
MedLine Citation:
PMID:  15111713     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The fluorescence emitted at 710 nm by Phaeodactylum tricornutum (F(710)) was characterized. Development of F(710) was found to be regulated by the quality of light needed for algal growth: weak red light absorbed mainly by Chl a induced its development, and weak blue-green light absorbed mainly by fucoxanthin and Chl c suppressed it. The difference spectra between cells grown under the two light conditions revealed two Chl a forms, absorption peaks of which were located at 692 nm (Chl a(692)) and at 703 nm (Chl a(703)), respectively, in red-light-grown cells. During cell growth under red light, the appearance and intensification of the emission correlated well with development of Chl a(692) and Chl a(703) suggesting that the two forms of Chl a are involved in the energy flow to F(710). A clear induction phenomenon characteristic of the PSII fluorescence was observed not only with the emission at 680 nm but also with F(710), indicating that F(710) is emitted by PSII Chl a. Development of F(710) under red light was sensitive to cycloheximide, indicating that the development of the energy flow to F(710) requires protein synthesis and that the emitter is installed in a protein encoded in the nuclear genome like the light-harvesting complex (LHC). Centrifugal fractionation of pigment-protein complexes revealed F(710) to be located at fractions slightly heavier than the major LHC. Development of F(710) was also found in red-light-grown cells of the diatom Nitzschia closterium.
Authors:
Yoshihiko Fujita; Kaori Ohki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plant & cell physiology     Volume:  45     ISSN:  0032-0781     ISO Abbreviation:  Plant Cell Physiol.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-04-27     Completed Date:  2004-07-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  392-7     Citation Subset:  IM    
Affiliation:
Department of Marine Bioscience, Fukui Prefectural University, Obama, Fukui, 917 Japan. fujitaky@mitene.or.jp
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MeSH Terms
Descriptor/Qualifier:
Cells, Cultured
Centrifugation, Density Gradient
Chlorophyll / chemistry,  radiation effects
Cycloheximide / pharmacology
Diatoms / chemistry*,  growth & development,  radiation effects
Fluorescence*
Light
Light-Harvesting Protein Complexes / chemistry,  metabolism
Spectrometry, Fluorescence
Temperature
Time Factors
Chemical
Reg. No./Substance:
0/Light-Harvesting Protein Complexes; 1406-65-1/Chlorophyll; 66-81-9/Cycloheximide

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