| [6S]-5-Methyltetrahydrofolate enhances folate status in rats fed growing-up milk. | |
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MedLine Citation:
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PMID: 19387717 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND AND AIMS: To investigate the efficiency of [6S]-5-methyltetrahydrofolate or Metafolin ([6S]-5-CH(3)-H(4)folate) on the recovery of folate status, we conducted a depletion-repletion rat model study using a growing-up milk as the folate carrier. METHODS: The effect of [6S]-5-CH(3)-H(4)folate was compared to that of folic acid (PGA or Pte-Glu), by feeding two groups of folate-depleted rats a diet of fortified growing-up milk containing either 1,000 microg/l (2.2655 micromol/l) of Pte-Glu or 1,041.91 microg/l (2.2655 micromol/l) of [6S]-5-CH(3)-H(4)folate over a 4-week period. At the end of the study, the folate concentration in plasma, erythrocytes and liver was measured to establish the folate status of the animals. The folate content was determined in the plasma and erythrocytes by a time-resolved fluoroimmunoassay method and in the liver by a HPLC method. RESULTS: Plasma, erythrocyte and liver folate concentrations were significantly (P < 0.001) lower after a depletion period in rats fed the folate-deficient diet compared to rats fed a control diet. The folate form used significantly influenced the folate concentration in erythrocytes and liver, but not in plasma, after the rats' body folate reserves were replenished by consuming the fortified growing-up milk. Thus, rats fed [6S]-5-CH(3)-H(4)folate-fortified growing-up milk showed significantly higher folate content in erythrocytes and liver (1,100.37 ng/ml and 4.22 microg/g, respectively), than did those fed Pte-Glu-fortified growing-up milk (827.71 ng/ml and 3.04 microg/g, respectively, in erythrocytes and liver). CONCLUSION: We conclude that the natural diastereomer [6S]-5-CH(3)-H(4)folate may adequately serve as an alternative to folic acid for the folate fortification of infant foods. |
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Authors:
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Darío Pérez-Conesa; Juan Francisco Haro-Vicente; Fernando Romero Braquehais; Gaspar Ros |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-04-23 |
Journal Detail:
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Title: European journal of nutrition Volume: 48 ISSN: 1436-6215 ISO Abbreviation: Eur J Nutr Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-09-04 Completed Date: 2009-12-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100888704 Medline TA: Eur J Nutr Country: Germany |
Other Details:
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Languages: eng Pagination: 365-71 Citation Subset: IM |
Affiliation:
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Nutrición y Bromatología, Facultad de Veterinaria, Universidad de Murcia, Campus Universitario de Espinardo, Murcia, Spain. dario@um.es |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Animals Animals, Newborn Body Weight Diet Erythrocytes / chemistry Folic Acid / analysis*, blood Folic Acid Deficiency* / blood, prevention & control Infant Formula / administration & dosage Limit of Detection Liver / chemistry Male Nutritional Status* Random Allocation Rats Rats, Sprague-Dawley Reproducibility of Results Stereoisomerism Tetrahydrofolates / administration & dosage* |
| Chemical | |
Reg. No./Substance:
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0/Tetrahydrofolates; 134-35-0/5-methyltetrahydrofolate; 59-30-3/Folic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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