Document Detail


A 610 kb YAC clone harbors 7 cM of tomato (Lycopersicon esculentum) DNA that includes the male sterile 14 gene and a hotspot for recombination.
MedLine Citation:
PMID:  8628247     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pollen development requires both sporophytic and gametophytic gene expression. We are using a map-based cloning technique to isolate sporophytic genes which, when mutant, cause pollen abortion and a male sterile (ms) phenotype in tomato (Lycopersicon esculentum). We have genetically characterized one ms locus (ms14) using RFLP analysis and identified flanking markers. High-resolution genomic physical mapping indicates that the ms14 locus is located in a approximately 300 kb region. We have identified a YAC clone with an insert size of approximately 610 kb that contains the ms14-linked markers, reflects the organization of the physical map and therefore most probably contains the ms14 gene. In addition, we present evidence that the relationship between physical and genetic distance in this chromosomal region changes abruptly from approximately 105-140 kb/cM to less than 24kb/cM, and suggest that the TG393-TG104 region is a hotspot for recombination.
Authors:
S W Gorman; D Banasiak; C Fairley; S McCormick
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Molecular & general genetics : MGG     Volume:  251     ISSN:  0026-8925     ISO Abbreviation:  Mol. Gen. Genet.     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1996-06-21     Completed Date:  1996-06-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0125036     Medline TA:  Mol Gen Genet     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  52-9     Citation Subset:  IM    
Affiliation:
Plant Gene Expression Center, USDA/ARS, Albany, CA USA.
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MeSH Terms
Descriptor/Qualifier:
Chromosome Mapping*
Chromosomes, Artificial, Yeast
Cloning, Molecular
DNA, Plant / analysis,  genetics
Genes, Plant*
Infertility
Lycopersicon esculentum / genetics*
Mutation*
Phenotype
Pollen
Polymorphism, Restriction Fragment Length
Recombination, Genetic*
Restriction Mapping
Chemical
Reg. No./Substance:
0/DNA, Plant

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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