Document Detail

A 610 kb YAC clone harbors 7 cM of tomato (Lycopersicon esculentum) DNA that includes the male sterile 14 gene and a hotspot for recombination.
MedLine Citation:
PMID:  8628247     Owner:  NLM     Status:  MEDLINE    
Pollen development requires both sporophytic and gametophytic gene expression. We are using a map-based cloning technique to isolate sporophytic genes which, when mutant, cause pollen abortion and a male sterile (ms) phenotype in tomato (Lycopersicon esculentum). We have genetically characterized one ms locus (ms14) using RFLP analysis and identified flanking markers. High-resolution genomic physical mapping indicates that the ms14 locus is located in a approximately 300 kb region. We have identified a YAC clone with an insert size of approximately 610 kb that contains the ms14-linked markers, reflects the organization of the physical map and therefore most probably contains the ms14 gene. In addition, we present evidence that the relationship between physical and genetic distance in this chromosomal region changes abruptly from approximately 105-140 kb/cM to less than 24kb/cM, and suggest that the TG393-TG104 region is a hotspot for recombination.
S W Gorman; D Banasiak; C Fairley; S McCormick
Related Documents :
1354637 - A high-resolution cytogenetic map of human chromosome 3: localization of 291 new cosmid...
17210077 - Update of the anopheles gambiae pest genome assembly.
15265077 - Comparative mapping of human chromosome 10 to pig chromosomes 10 and 14.
11306817 - Distribution of hox genes in the chicken genome reveals a new segment of conservation b...
9450847 - Ullrich-turner syndrome: seven pregnancies in an apparent 45,x woman.
6579837 - Acquired partial deletions of the long arm of chromosome 5 in hematologic disorders.
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Molecular & general genetics : MGG     Volume:  251     ISSN:  0026-8925     ISO Abbreviation:  Mol. Gen. Genet.     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1996-06-21     Completed Date:  1996-06-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0125036     Medline TA:  Mol Gen Genet     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  52-9     Citation Subset:  IM    
Plant Gene Expression Center, USDA/ARS, Albany, CA USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Chromosome Mapping*
Chromosomes, Artificial, Yeast
Cloning, Molecular
DNA, Plant / analysis,  genetics
Genes, Plant*
Lycopersicon esculentum / genetics*
Polymorphism, Restriction Fragment Length
Recombination, Genetic*
Restriction Mapping
Reg. No./Substance:
0/DNA, Plant

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Rhizobium nodulation protein NodA is a host-specific determinant of the transfer of fatty acids in N...
Next Document:  An asexual fungus has the potential for sexual development.