| 6-phospho-D-gluconate dehydrogenase from Pseudomonas fluorescens. Properties and subunit structure. | |
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MedLine Citation:
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PMID: 6402366 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. The 6-phospho-D-gluconate dehydrogenase (decarboxylating) (EC 1.1.1.44) from Pseudomonas fluorescens, a B-side stereospecific enzyme, is active with both NAD+ and NADP+, having a specific activity of the homogeneous enzyme of 121 mumols NADH and 23 mumols NADPH, respectively, formed min-1 mg protein-1. The pI of the native enzyme is 4.62, the pH optimum is about 8.2. 2. The molecular weight of the native enzyme has been determined to be 126000 by sedimentation equilibrium studies. The molecular weight of the polypeptide chains composing the enzyme has been found to be 32000 by dodecylsulfate/polyacrylamide gel electrophoresis and 31000 by sedimentation equilibrium studies in presence of 6 M guanidine hydrochloride. The native enzyme is composed of four polypeptide chains. 3. Reacting enzyme centrifugation studies gave at pH 8.2 a sedimentation coefficient s20, w of 8.04 S and a diffusion coefficient D20, w of 6.56 F, resulting in a molecular weight of 115000 for the catalytically active form. Thus, the enzyme is active as the tetramer. So far the enzyme from P. fluorescens is the sole 6-phospho-D-gluconate dehydrogenase (decarboxylating) composed of four polypeptide chains. |
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Authors:
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C Stournaras; P Maurer; G Kurz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of biochemistry / FEBS Volume: 130 ISSN: 0014-2956 ISO Abbreviation: Eur. J. Biochem. Publication Date: 1983 Feb |
Date Detail:
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Created Date: 1983-04-07 Completed Date: 1983-04-07 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0107600 Medline TA: Eur J Biochem Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 391-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Bacterial Proteins / isolation & purification* Chemical Phenomena Chemistry Isoelectric Point Phosphogluconate Dehydrogenase / isolation & purification* Pseudomonas fluorescens / enzymology* Ultracentrifugation |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Bacterial Proteins; EC 1.1.1.43/Phosphogluconate Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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