Document Detail


6-phospho-D-gluconate dehydrogenase from Pseudomonas fluorescens. Properties and subunit structure.
MedLine Citation:
PMID:  6402366     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. The 6-phospho-D-gluconate dehydrogenase (decarboxylating) (EC 1.1.1.44) from Pseudomonas fluorescens, a B-side stereospecific enzyme, is active with both NAD+ and NADP+, having a specific activity of the homogeneous enzyme of 121 mumols NADH and 23 mumols NADPH, respectively, formed min-1 mg protein-1. The pI of the native enzyme is 4.62, the pH optimum is about 8.2. 2. The molecular weight of the native enzyme has been determined to be 126000 by sedimentation equilibrium studies. The molecular weight of the polypeptide chains composing the enzyme has been found to be 32000 by dodecylsulfate/polyacrylamide gel electrophoresis and 31000 by sedimentation equilibrium studies in presence of 6 M guanidine hydrochloride. The native enzyme is composed of four polypeptide chains. 3. Reacting enzyme centrifugation studies gave at pH 8.2 a sedimentation coefficient s20, w of 8.04 S and a diffusion coefficient D20, w of 6.56 F, resulting in a molecular weight of 115000 for the catalytically active form. Thus, the enzyme is active as the tetramer. So far the enzyme from P. fluorescens is the sole 6-phospho-D-gluconate dehydrogenase (decarboxylating) composed of four polypeptide chains.
Authors:
C Stournaras; P Maurer; G Kurz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  130     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1983 Feb 
Date Detail:
Created Date:  1983-04-07     Completed Date:  1983-04-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  391-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Bacterial Proteins / isolation & purification*
Chemical Phenomena
Chemistry
Isoelectric Point
Phosphogluconate Dehydrogenase / isolation & purification*
Pseudomonas fluorescens / enzymology*
Ultracentrifugation
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Bacterial Proteins; EC 1.1.1.43/Phosphogluconate Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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