Document Detail

6'-Sulfo sialyl Lex but not 6-sulfo sialyl Lex expressed on the cell surface supports L-selectin-mediated adhesion.
MedLine Citation:
PMID:  8910289     Owner:  NLM     Status:  MEDLINE    
In order to determine if a sulfated oligosaccharide on the cell surface can function as an L-selectin ligand, a novel approach for in vitro transfer of oligosaccharides was utilized (Srivastava, G., Kaun, K. J., Hindsgaul, O., and Palcic, M. M. (1992) J. Biol. Chem. 267, 22356-22361). CHO cells were incubated with synthetic 6'-sulfo sialyl Lex, NeuNAcalpha2-->3(sulfate-6)Galbeta1-->4(Fucalpha1-->3) GlcNAc or 6-sulfo sialyl Lex, NeuNAcalpha2-->3Galbeta1-->4[(Fucalpha1-->3)sulfate--> 6GlcNAc] oligosaccharide linked to C-6 of a fucose residue in GDP-fucose and a milk fucosyltransferase. The resultant CHO cells expressing 6'-sulfo sialyl Lex or 6-sulfo sialyl Lex on their cell surface were tested for adhesion to E-selectin and L-selectin chimeric proteins coated on plates. The results indicate that 6'-sulfo sialyl Lex supports L-selectin-mediated adhesion much better than sialyl Lex similarly tagged on the cell surface. In contrast, 6-sulfo sialyl Lex containing a sulfate group on the N-acetylglucosamine residue did not support adhesion with either selectin. These combined results suggest that 6'-sulfo sialyl Lex is a much better ligand than sialyl Lex oligosaccharide for L-selectin.
S Tsuboi; Y Isogai; N Hada; J K King; O Hindsgaul; M Fukuda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  271     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1996-12-26     Completed Date:  1996-12-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  27213-6     Citation Subset:  IM    
Glycobiology Program, La Jolla Cancer Research Center, The Burnham Institute, La Jolla, California 92037, USA.
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MeSH Terms
CHO Cells
Carbohydrate Conformation
Carbohydrate Sequence
Cell Adhesion*
Cell Membrane / physiology
Fucosyltransferases / metabolism
Guanosine Diphosphate Fucose / metabolism
L-Selectin / metabolism*
Milk / enzymology
Molecular Sequence Data
Oligosaccharides / biosynthesis,  chemistry,  metabolism*
Recombinant Fusion Proteins / metabolism
Grant Support
Reg. No./Substance:
0/Oligosaccharides; 0/Recombinant Fusion Proteins; 0/sialyl-2-3-(6'-sulfo)galactosyl-1-4-(fucopyranosyl-1-3)-N-acetylglucosamine; 126880-86-2/L-Selectin; 15839-70-0/Guanosine Diphosphate Fucose; EC 2.4.1.-/Fucosyltransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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