Document Detail


6'-Sulfo sialyl Lex but not 6-sulfo sialyl Lex expressed on the cell surface supports L-selectin-mediated adhesion.
MedLine Citation:
PMID:  8910289     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to determine if a sulfated oligosaccharide on the cell surface can function as an L-selectin ligand, a novel approach for in vitro transfer of oligosaccharides was utilized (Srivastava, G., Kaun, K. J., Hindsgaul, O., and Palcic, M. M. (1992) J. Biol. Chem. 267, 22356-22361). CHO cells were incubated with synthetic 6'-sulfo sialyl Lex, NeuNAcalpha2-->3(sulfate-6)Galbeta1-->4(Fucalpha1-->3) GlcNAc or 6-sulfo sialyl Lex, NeuNAcalpha2-->3Galbeta1-->4[(Fucalpha1-->3)sulfate--> 6GlcNAc] oligosaccharide linked to C-6 of a fucose residue in GDP-fucose and a milk fucosyltransferase. The resultant CHO cells expressing 6'-sulfo sialyl Lex or 6-sulfo sialyl Lex on their cell surface were tested for adhesion to E-selectin and L-selectin chimeric proteins coated on plates. The results indicate that 6'-sulfo sialyl Lex supports L-selectin-mediated adhesion much better than sialyl Lex similarly tagged on the cell surface. In contrast, 6-sulfo sialyl Lex containing a sulfate group on the N-acetylglucosamine residue did not support adhesion with either selectin. These combined results suggest that 6'-sulfo sialyl Lex is a much better ligand than sialyl Lex oligosaccharide for L-selectin.
Authors:
S Tsuboi; Y Isogai; N Hada; J K King; O Hindsgaul; M Fukuda
Related Documents :
11091549 - Adhesion of human breast cancer cells to vascular endothelium mediated by sialyl lewis ...
22588089 - Mussel-inspired load bearing metal-polymer glues.
12686549 - Expression patterns of alpha 2,3-sialyltransferases and alpha 1,3-fucosyltransferases d...
12369089 - Design and synthesis of sialyl lewis(x) mimics as e- and p-selectin inhibitors.
12163379 - Glycosylation might provide endothelial zip codes for organ-specific leukocyte traffic ...
11935309 - Transfection of the nm23-h1 gene into human hepatocarcinoma cell line inhibits the expr...
20825419 - Role of interleukin-17 in lymphangiogenesis in non-small-cell lung cancer: enhanced pro...
3267699 - Cell adhesion to hydroxyl groups of a monolayer film.
10704369 - Rho and rac exert antagonistic functions on spreading of macrophage-derived multinuclea...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  271     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1996-12-26     Completed Date:  1996-12-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  27213-6     Citation Subset:  IM    
Affiliation:
Glycobiology Program, La Jolla Cancer Research Center, The Burnham Institute, La Jolla, California 92037, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
CHO Cells
Carbohydrate Conformation
Carbohydrate Sequence
Cattle
Cell Adhesion*
Cell Membrane / physiology
Cricetinae
Fucosyltransferases / metabolism
Guanosine Diphosphate Fucose / metabolism
L-Selectin / metabolism*
Milk / enzymology
Molecular Sequence Data
Oligosaccharides / biosynthesis,  chemistry,  metabolism*
Recombinant Fusion Proteins / metabolism
Transfection
Grant Support
ID/Acronym/Agency:
P01 CA71932/CA/NCI NIH HHS; R01 CA48737/CA/NCI NIH HHS; R37 CA33000/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Oligosaccharides; 0/Recombinant Fusion Proteins; 0/sialyl-2-3-(6'-sulfo)galactosyl-1-4-(fucopyranosyl-1-3)-N-acetylglucosamine; 126880-86-2/L-Selectin; 15839-70-0/Guanosine Diphosphate Fucose; EC 2.4.1.-/Fucosyltransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The GTPase-activating protein RGS4 stabilizes the transition state for nucleotide hydrolysis.
Next Document:  Inactivation of a novel neuropeptide Y/peptide YY receptor gene in primate species.