| Δ(6)-Desaturase from Mortierella alpina: cDNA cloning, expression, and phylogenetic analysis. | |
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MedLine Citation:
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PMID: 21691840 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The open reading frame of the Δ(6)-desaturase gene was isolated from Mortierella alpina W15 and the gene was cloned into a pPIC3.5K vector. The vector was transformed into Pichia pastoris GS115 and expression was induced with methanol. The Δ(6)-desaturase expressed in P. pastoris GS115 catalyzed the conversion of linoleic acid to γ-linolenic acid but not the conversion of α-linolenic acid to octadecatetraenoic acid. The results indicate that the Δ(6)-desaturase gene from M. alpina W15 has substrate specificity in different organisms. Phylogenetic analysis revealed that Δ(6)-desaturase genes can be divided into four monophyletic groups. This work paves the way for further study of the functions of Δ(6)-desaturase in fatty acid metabolism and its three-dimensional structure. |
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Authors:
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Jianmin Liu; Dianrong Li; Yongtai Yin; Hao Wang; Maoteng Li; Longjiang Yu |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-6-21 |
Journal Detail:
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Title: Biotechnology letters Volume: - ISSN: 1573-6776 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-6-21 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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