| 5S rRNA modification in the hyperthermophilic archaea Sulfolobus solfataricus and Pyrodictium occultum. | |
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MedLine Citation:
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PMID: 8422966 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The 5S rRNAs from Sulfolobus solfataricus and Pyrodictium occultum were digested to nucleosides and analyzed using directly-combined HPLC/mass spectrometry. P. occultum 5S rRNA contains two modified nucleoside species, N4-acetylcytidine (ac4C) and N4-acetyl-2'-O-methylcytidine (ac4Cm). Oligonucleotides were generated from P. occultum 5S rRNA by RNase T1 hydrolysis, and their molecular weights were determined using electrospray mass spectrometry and compared with those predicted from the P. occultum 5S RNA gene sequence. Deviation in mass between expected and observed molecular weights permitted ac4Cm to be located at position 35, in the nonanucleotide CAA-CACC[ac4Cm]G, and the ac4C in one or both of two (C,U)G trinucleotides. 2'-O-Methylcytidine is unambiguously characterized in S. solfataricus 5S rRNA, confirming earlier tentative assignments at the analogous sequence position (Stahl, D.A., Luehrsen, K.R., Woese, C.R., and Pace, N.R. (1981) Nucleic Acids Res., Vol. 9, pp. 6129-6137; Dams, E., Londei, P., Cammarano, P., Vandenberghe, A., and De Wachter, R. (1983) Nucleic Acids Res. Vol. 11, pp. 4667-4676). Potential effects of the presence of ac4C and ac4Cm on thermal stabilization of 5S rRNA in thermophiles are discussed. |
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Authors:
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E Bruenger; J A Kowalak; Y Kuchino; J A McCloskey; H Mizushima; K O Stetter; P F Crain |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: FASEB journal : official publication of the Federation of American Societies for Experimental Biology Volume: 7 ISSN: 0892-6638 ISO Abbreviation: FASEB J. Publication Date: 1993 Jan |
Date Detail:
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Created Date: 1993-02-23 Completed Date: 1993-02-23 Revised Date: 2012-02-15 |
Medline Journal Info:
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Nlm Unique ID: 8804484 Medline TA: FASEB J Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 196-200 Citation Subset: IM |
Affiliation:
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Department of Medicinal Chemistry, University of Utah, Salt Lake City 84112. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Archaea
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chemistry* Base Sequence Cytidine / analogs & derivatives*, analysis Hot Temperature Molecular Sequence Data Nucleic Acid Conformation Nucleic Acid Denaturation Nucleosides / analysis* RNA, Ribosomal, 5S / chemistry*, drug effects Ribonuclease T1 / pharmacology Sulfolobus |
| Grant Support | |
ID/Acronym/Agency:
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GM 29812/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Nucleosides; 0/RNA, Ribosomal, 5S; 2140-61-6/5-methylcytidine; 65-46-3/Cytidine; EC 3.1.27.3/Ribonuclease T1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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