| 5HT-1A receptors and anxiety-like behaviours: studies in rats with constitutionally upregulated/downregulated serotonin transporter. | |
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MedLine Citation:
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PMID: 20457184 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Altered activity of brain serotonergic (5HT) system has been implicated in a wide range of behaviours and behavioural disorders, including anxiety. Functioning of 5HT-1A receptor has been suggested as a modulator of emotional balance in both, normal and pathological forms of anxiety. Here, we studied serotonergic modulation of anxiety-like behaviour using a genetic rat model with constitutional differences in 5HT homeostasis, named Wistar-Zagreb 5HT (WZ-5HT) rats. The model, consisting of high-5HT and low-5HT sublines, was developed by selective breeding of animals for extreme activities of peripheral (platelet) 5HT transporter, but selection process had affected also central 5HT homeostasis, as evidenced from neurochemical and behavioural studies. Anxiety-like behaviour in WZ-5HT rats was evaluated by two commonly used paradigms: open field and elevated-plus maze. The involvement of 5HT-1A receptors in behavioural response was assessed by measuring mRNA expression in cell bodies (raphe nuclei) and projection regions (frontal cortex, hippocampus) by use of RT-PCR and in situ hybridization, and by measuring functionality of cortical 5HT-1A receptors by use of [(3)H]8-OH-DPAT radioligand binding. Animals from the high-5HT subline exhibit increased anxiety-like behaviour and decreased exploratory activity when exposed to novel environment. No measurable differences in constitutional (baseline) functionality or expression of 5HT-1A receptors between sublines were found. The results support contribution of increased serotonergic functioning to the anxiety-like behaviour. They also validate the high-5HT subline of WZ-5HT rats as a potential model to study mechanisms of anxiety, especially of its nonpathological form, while the low-5HT subline may be useful to model sensation seeking phenotype. |
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Authors:
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Tatjana Bordukalo-Niksic; Gordana Mokrovic; Jasminka Stefulj; Marko Zivin; Branimir Jernej; Lipa Cicin-Sain |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-08 |
Journal Detail:
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Title: Behavioural brain research Volume: 213 ISSN: 1872-7549 ISO Abbreviation: Behav. Brain Res. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-07-12 Completed Date: 2010-10-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8004872 Medline TA: Behav Brain Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 238-45 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Laboratory of Neurochemistry and Molecular Neurobiology, Department of Molecular Biology, Rudjer Boskovic Institute, Bijenicka 54, Zagreb, Croatia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anxiety / genetics, metabolism* Disease Models, Animal Down-Regulation / physiology* Exploratory Behavior / physiology* Female Frontal Lobe / metabolism Hippocampus / metabolism Male Maze Learning / physiology* Raphe Nuclei / metabolism Rats Rats, Inbred Strains / genetics* Receptor, Serotonin, 5-HT1A / metabolism* Serotonin Plasma Membrane Transport Proteins / biosynthesis*, genetics Up-Regulation / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Serotonin Plasma Membrane Transport Proteins; 112692-38-3/Receptor, Serotonin, 5-HT1A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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