| ABHD5/CGI-58 facilitates the assembly and secretion of apolipoprotein B lipoproteins by McA RH7777 rat hepatoma cells. | |
| | |
MedLine Citation:
|
PMID: 19211039 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Lipolysis of stored triacylglycerols provides lipid precursors for the assembly of apolipoprotein B (apoB) lipoproteins in hepatocytes. Abhydrolase domain containing 5 (ABHD5) is expressed in liver and facilitates the lipolysis of triacylglycerols. To study the function of ABHD5 in lipoprotein secretion, we silenced the expression of ABHD5 in McA RH7777 cells using RNA interference and studied the metabolism of lipids and secretion of apoB lipoproteins. McA RH7777 cells deficient in ABHD5 secreted reduced amounts of apoB, triacylglycerols, and cholesterol esters. Detailed analysis of liquid chromatography-mass spectrometry data for the molecular species of secreted triacylglycerols revealed that deficiency of ABHD5 significantly reduced secretion of triacylglycerols containing oleate, even when oleate was supplied in the culture medium; the ABHD5-deficient cells partially compensated by secreting higher levels of triacylglycerols containing saturated fatty acids. In experiments tracking the metabolism of [(14)C]oleate, silencing of ABHD5 reduced lipolysis of cellular triacylglycerols and incorporation of intermediates derived from stored lipids into secreted triacylglycerols and cholesterol esters. In contrast, the incorporation of exogenous oleate into secreted triacylglycerols and cholesterol esters was unaffected by deficiency of ABHD5. These findings suggest that ABHD5 facilitates the use of lipid intermediates derived from lipolysis of stored triacylglycerols for the assembly of lipoproteins. |
| | |
Authors:
|
Jorge M Caviglia; Janet D Sparks; Nikhil Toraskar; Anita M Brinker; Terry C Yin; Joseph L Dixon; Dawn L Brasaemle |
Related Documents
:
|
7575449 - Modulation of hepatic apolipoprotein b, 3-hydroxy-3-methylglutaryl-coa reductase and lo... 16723719 - Aggressive very low-density lipoprotein (vldl) and ldl lowering by gene transfer of the... 6621369 - Preliminary report increased transport of intermediate density lipoprotein (ldl) with c... 8978489 - Hypocholesterolemic actions of atorvastatin are associated with alterations on hepatic ... 10412729 - New insights into lipid metabolism in the nephrotic syndrome. 21739249 - Dairy milk fat augments paclitaxel therapy to suppress tumour metastasis in mice, and p... 2156419 - A case-control study of diet and rectal cancer in western new york. 18494169 - Effectiveness of the mediterranean diet in the elderly. 6448279 - Effects of chronic ascorbic acid deficiency on guinea pig lysosomal hydrolase activities. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-01-13 |
Journal Detail:
|
Title: Biochimica et biophysica acta Volume: 1791 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2009 Mar |
Date Detail:
|
Created Date: 2009-02-27 Completed Date: 2009-04-28 Revised Date: 2011-09-26 |
Medline Journal Info:
|
Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 198-205 Citation Subset: IM |
Affiliation:
|
Rutgers Center for Lipid Research, Rutgers, The State University of New Jersey, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
1-Acylglycerol-3-Phosphate O-Acyltransferase Animals Apolipoproteins B / metabolism* Carcinoma, Hepatocellular / metabolism Carrier Proteins / physiology* Chromatography, Liquid Esterases / physiology* Lipids / analysis Lipoproteins / genetics, metabolism* Mass Spectrometry RNA, Small Interfering / pharmacology Rats Triglycerides / secretion Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
|
R01 DK050376-09/DK/NIDDK NIH HHS; R01 DK054797-08/DK/NIDDK NIH HHS; R01 DK50376/DK/NIDDK NIH HHS; R01 DK54797/DK/NIDDK NIH HHS; R01 HL047586/HL/NHLBI NIH HHS; R01 HL047586-12/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Apolipoproteins B; 0/CGI-58 protein, rat; 0/Carrier Proteins; 0/Lipids; 0/Lipoproteins; 0/RNA, Small Interfering; 0/Triglycerides; EC 2.3.1.51/1-Acylglycerol-3-Phosphate O-Acyltransferase; EC 2.3.1.51/Abhd5 protein, mouse; EC 3.1.-/Esterases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Participation in aflatoxin biosynthesis by a reductase enzyme encoded by vrdA gene outside the aflat...
Next Document: Inhibition of acetylcholinesterase by metabolites of copper pyrithione (CuPT) and its possible invol...