Document Detail

5,7-Dihydroxytryptamine uptake discriminates living serotonergic cells from dopaminergic cells in rat midbrain culture.
MedLine Citation:
PMID:  8577399     Owner:  NLM     Status:  MEDLINE    
Dissociated cells from embryonic rat midbrain develop in dissociated culture into glutamatergic, GABAergic and aminergic cells. The autofluorescent serotonin analogue, 5,7-dihydroxytryptamine (5,7-DHT), is taken up by a small population of cells that is immunoreactive to 5-hydroxytryptamine. Tyrosine hydroxylase-immunoreactive cells do not accumulate 5,7-DHT. 5,7-DHT uptake, therefore, is well suited for the identification of living serotonergic cells and their discrimination from dopaminergic cells.
J Rohrbacher; K Krieglstein; S Honerkamp; A Lewen; U Misgeld
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience letters     Volume:  199     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  1995 Oct 
Date Detail:
Created Date:  1996-03-13     Completed Date:  1996-03-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  207-10     Citation Subset:  IM    
I. Physiologisches Institut, Universität Heidelberg, Germany.
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MeSH Terms
5,7-Dihydroxytryptamine / pharmacokinetics*
Animals, Newborn
Cells, Cultured
Dopamine / metabolism*
Mesencephalon / metabolism*
Neurons / metabolism
Serotonin / metabolism*
Reg. No./Substance:
31363-74-3/5,7-Dihydroxytryptamine; 50-67-9/Serotonin

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