| 5,7-Dihydroxytryptamine uptake discriminates living serotonergic cells from dopaminergic cells in rat midbrain culture. | |
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MedLine Citation:
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PMID: 8577399 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dissociated cells from embryonic rat midbrain develop in dissociated culture into glutamatergic, GABAergic and aminergic cells. The autofluorescent serotonin analogue, 5,7-dihydroxytryptamine (5,7-DHT), is taken up by a small population of cells that is immunoreactive to 5-hydroxytryptamine. Tyrosine hydroxylase-immunoreactive cells do not accumulate 5,7-DHT. 5,7-DHT uptake, therefore, is well suited for the identification of living serotonergic cells and their discrimination from dopaminergic cells. |
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Authors:
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J Rohrbacher; K Krieglstein; S Honerkamp; A Lewen; U Misgeld |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neuroscience letters Volume: 199 ISSN: 0304-3940 ISO Abbreviation: Neurosci. Lett. Publication Date: 1995 Oct |
Date Detail:
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Created Date: 1996-03-13 Completed Date: 1996-03-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7600130 Medline TA: Neurosci Lett Country: IRELAND |
Other Details:
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Languages: eng Pagination: 207-10 Citation Subset: IM |
Affiliation:
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I. Physiologisches Institut, Universität Heidelberg, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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5,7-Dihydroxytryptamine
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pharmacokinetics* Animals Animals, Newborn Cells, Cultured Dopamine / metabolism* Immunohistochemistry Mesencephalon / metabolism* Neurons / metabolism Rats Serotonin / metabolism* |
| Chemical | |
Reg. No./Substance:
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31363-74-3/5,7-Dihydroxytryptamine; 50-67-9/Serotonin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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