Document Detail

5,5'-Dimethyloxazolidine-2,4-dione is a strong inducer of differentiation of human promyelocytic leukemia (HL-60) cells.
MedLine Citation:
PMID:  2766039     Owner:  NLM     Status:  MEDLINE    
5,5'-Dimethyloxazolidine-2,4-dione (DMO), a weak non-metabolizable acid, is commonly utilized for determining intracellular pH. In these studies, DMO was tested as an inducer of differentiation on the basis that its uptake and subsequent dissociation might transiently raise intracellular pH and activate ion-fluxes critical for triggering maturation. After 5 days of exposure to 40 mM DMO, greater than 60% of HL-60 cells displayed phenotypic and functional changes characteristic of mature granulocytes. As with other inducers of HL-60 cell differentiation, commitment to differentiation required culture in the presence of DMO for more than 24 h, indicating that if transient effects on pH or ion-fluxes occurred, they were not sufficient to trigger this process. DMO was either weak or inactive as an inducer of murine erythroleukemia cell (FLC) differentiation. Although other weak acids and bases triggered differentiation of both HL-60 cells and FLC, the spectrum of response differed markedly between the two lines. These results suggest that: (1) a number of common buffering agents have the potential to alter cell phenotype, and (2) their effects must be evaluated for each individual cell type.
T M Calderon; N Schneiderman; J Michl; J K Christman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell differentiation and development : the official journal of the International Society of Developmental Biologists     Volume:  26     ISSN:  0922-3371     ISO Abbreviation:  Cell Differ. Dev.     Publication Date:  1989 May 
Date Detail:
Created Date:  1989-10-04     Completed Date:  1989-10-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8811335     Medline TA:  Cell Differ Dev     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  181-90     Citation Subset:  IM    
Department of Biochemistry, Mount Sinai School of Medicine, New York, NY 10021.
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MeSH Terms
Cell Division / drug effects
Cell Line
Cell Transformation, Neoplastic / drug effects
Dimethadione / pharmacology*
Hydrogen-Ion Concentration
Leukemia, Erythroblastic, Acute / pathology,  physiopathology
Leukemia, Promyelocytic, Acute / pathology*,  physiopathology
Oxazoles / pharmacology*
Tumor Cells, Cultured / drug effects
Grant Support
Reg. No./Substance:
0/Oxazoles; 695-53-4/Dimethadione

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