Document Detail


A 54-kDa fragment of the Poly(A)-specific ribonuclease is an oligomeric, processive, and cap-interacting Poly(A)-specific 3' exonuclease.
MedLine Citation:
PMID:  10801819     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have previously identified a HeLa cell 3' exonuclease specific for degrading poly(A) tails of mRNAs. Here we report on the purification and identification of a calf thymus 54-kDa polypeptide associated with a similar 3' exonuclease activity. The 54-kDa polypeptide was shown to be a fragment of the poly(A)-specific ribonuclease 74-kDa polypeptide. The native molecular mass of the nuclease activity was estimated to be 180-220 kDa. Protein/protein cross-linking revealed an oligomeric structure, most likely consisting of three subunits. The purified nuclease activity released 5'-AMP as the reaction product and degraded poly(A) in a highly processive fashion. The activity required monovalent cations and was dependent on divalent metal ions. The RNA substrate requirement was investigated, and it was found that the nuclease was highly poly(A)-specific and that only 3' end-located poly(A) was degraded by the activity. RNA substrates capped with m(7)G(5')ppp(5')G were more efficiently degraded than noncapped RNA substrates. Addition of free m(7)G(5')ppp(5')G cap analogue inhibited poly(A) degradation in vitro, suggesting a functional link between the RNA 5' end cap structure and poly(A) degradation at the 3' end of the RNA.
Authors:
J Martinez; Y G Ren; A C Thuresson; U Hellman; J Astrom; A Virtanen
Related Documents :
15201049 - Role of an active site guanine in hairpin ribozyme catalysis probed by exogenous nucleo...
1474579 - Structural requirements for the processing of escherichia coli 5 s ribosomal rna by rna...
2438129 - Formation of mrna 3' termini: stability and dissociation of a complex involving the aau...
21050179 - A new rnase sheds light on the rnase/angiogenin subfamily from zebrafish.
22835149 - Insertion of a myc-tag within α-dystroglycan domains improves its biochemical and micro...
11927599 - Potassium functionally replaces the second lysine of the kmsks signature sequence in hu...
22609859 - Structure of mammalian poly(adp-ribose) glycohydrolase reveals a flexible tyrosine clas...
19124459 - Analysis of transient and catalytic desosamine-binding pockets in cytochrome p-450 pikc...
123759 - Subunit dissociation and unfolding of rabbit muscle phosphofructokinase by guanidine by...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  275     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2000-09-07     Completed Date:  2000-09-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  24222-30     Citation Subset:  IM    
Affiliation:
Department of Cell and Molecular Biology, Uppsala University, Box 596, SE-751 24 Uppsala, Sweden.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adenosine Monophosphate / metabolism
Animals
Cattle
Chromatography, Affinity / methods
Exoribonucleases / isolation & purification,  metabolism*
Movement
Peptide Fragments / metabolism
Protein Binding
Protein Structure, Quaternary
RNA Caps / metabolism*
RNA Processing, Post-Transcriptional
Substrate Specificity
Thymus Gland / enzymology*
Chemical
Reg. No./Substance:
0/Peptide Fragments; 0/RNA Caps; 61-19-8/Adenosine Monophosphate; EC 3.1.-/Exoribonucleases; EC 3.1.13.4/poly(A)-specific ribonuclease

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Characterization of the interaction between zyxin and members of the Ena/vasodilator-stimulated phos...
Next Document:  Essential role of dynamin in internalization of M2 muscarinic acetylcholine and angiotensin AT1A rec...