| 51Cr-RBCs and 125I-albumin as markers to estimate lymph drainage of the peritoneal cavity in sheep. | |
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MedLine Citation:
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PMID: 8175601 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The purpose of this study was to compare the use of 125I-labeled human serum albumin (125I-HSA) and autologous 51Cr-labeled red blood cells (51Cr-RBCs) as lymph flow markers to estimate lymph drainage of the peritoneal cavity in conscious sheep. In one group, we assessed lymph drainage from the appearance of intraperitoneally administered tracer in the bloodstream. To determine distribution of drainage into discrete lymph compartments, in a second group of studies, lymph was collected from the caudal mediastinal lymph node and the thoracic duct, both of which are involved in lymphatic drainage of the ovine peritoneal cavity. Ringer lactate solution (50 ml/kg) containing 8-10 microCi each of 125I-HSA and 51Cr-RBCs was infused into the peritoneal cavity. Lymph drainage was calculated by dividing the change in mass of tracer in the blood or lymph compartments by the average intraperitoneal tracer concentration. In noncannulated animals, lymph drainage averaged over 6 h was higher with 125I-HSA as tracer (1.35 +/- 0.12 vs. 0.62 +/- 0.19 ml.h-1.kg-1 with 51Cr-RBCs). A similar pattern was noted in terms of drainage into the caudal lymphatic (0.89 +/- 0.23 and 0.52 +/- 0.19 ml.h-1.kg-1 with 125I-HSA and 51Cr-RBCs, respectively) and thoracic duct (0.16 +/- 0.06 and 0.05 +/- 0.02 ml.h-1.kg-1 with 125I-HSA and 51Cr-RBCs, respectively). Analysis of 125I-HSA and 51Cr-RBC concentrations in lymph and intraperitoneal fluid suggested sieving of RBCs at the diaphragmatic stomata or lymph nodes. Using 125I-HSA as tracer and combining data from noncannulated and cannulated sheep, we estimated peritoneal lymph drainage to be 1.35 ml.h-1.kg-1, with 66% of this flow drained by the caudal vessel, 22% by the parasternal pathway (right lymph duct), and 12% by the thoracic duct. |
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Authors:
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Z Y Yuan; H Rodela; J B Hay; D Oreopoulos; M G Johnston |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: 76 ISSN: 8750-7587 ISO Abbreviation: J. Appl. Physiol. Publication Date: 1994 Feb |
Date Detail:
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Created Date: 1994-06-06 Completed Date: 1994-06-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 867-74 Citation Subset: IM |
Affiliation:
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Trauma Research Program, Sunnybrook Health Science Centre, Toronto, Ontario, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Catheterization Chromium Radioisotopes* Erythrocytes* Female Humans Iodine Radioisotopes* Isotonic Solutions / pharmacology Lymph / metabolism* Mediastinum Peritoneal Cavity* Serum Albumin* Sheep Thoracic Duct / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Chromium Radioisotopes; 0/Iodine Radioisotopes; 0/Isotonic Solutions; 0/Serum Albumin; 8026-10-6/Ringer's solution |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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