Document Detail


5' flanking sequences of the murine adenosine deaminase gene direct expression of a reporter gene to specific prenatal and postnatal tissues in transgenic mice.
MedLine Citation:
PMID:  1618849     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adenosine deaminase (ADA), an enzyme of purine metabolism, is highly expressed in four tissues of the mouse: the maternal decidua, the fetal placenta, the keratinizing epithelium of the upper alimentary tract (tongue, esophagus, and forestomach), and the absorptive epithelium of the proximal small intestine. ADA is produced at relatively low levels in all other tissues. To identify genetic elements that direct appropriate prenatal and postnatal expression of the ADA gene, a segment of DNA including the ADA promoter and 6.4 kilobases of the adjacent 5' flanking region was tested for the ability to direct the expression of a reporter gene in transgenic mice. In seven lines of transgenic mice studied, this construct directed high levels of reporter gene expression in the placenta and forestomach and exhibited correct developmental regulation in these tissues. This construct failed to direct significant reporter gene expression to either the maternal decidua or the proximal small intestine. Thus, different gene regulatory elements are required to target high expression to the four tissues characterized by high levels of ADA.
Authors:
J H Winston; G R Hanten; P A Overbeek; R E Kellems
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  267     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1992 Jul 
Date Detail:
Created Date:  1992-08-05     Completed Date:  1992-08-05     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  13472-9     Citation Subset:  IM    
Affiliation:
Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Deaminase / genetics*
Animals
Blotting, Southern
Chloramphenicol O-Acetyltransferase / metabolism
Embryo, Mammalian / enzymology
Esophagus / enzymology
Female
Gene Expression
Male
Mice
Mice, Transgenic
Placenta / enzymology
Plasmids
Promoter Regions, Genetic
RNA, Messenger / metabolism
Stomach / enzymology
Tongue / enzymology
Grant Support
ID/Acronym/Agency:
DK46207/DK/NIDDK NIH HHS; GM30204/GM/NIGMS NIH HHS; HD24064/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Messenger; EC 2.3.1.28/Chloramphenicol O-Acetyltransferase; EC 3.5.4.4/Adenosine Deaminase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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