| 5 alpha-cholest-8(14)-en-3 beta-ol-15-one, a potent regulator of cholesterol metabolism: occurrence in rat skin. | |
| | |
MedLine Citation:
|
PMID: 3183517 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
5 alpha-Cholest-8(14)-en-3 beta-ol-15-one is a potent inhibitor of cholesterol biosynthesis which has significant hypocholesterolemic activity upon oral administration to animals. Described herein are the results of experiments that indicate the presence of the 15-ketosterol in rat skin. The 15-ketosterol was, after purification by medium pressure liquid chromatography on Lichroprep RP-8 columns, thin-layer chromatography on silica gel G, and reverse phase high performance liquid chromatography, characterized by gas-liquid chromatography-mass spectrometry in the form of its trimethylsilyl ether derivative. The use of an internal standard containing both tritium and deuterium permitted the determination of the levels of the 15-ketosterol by mass fragmentography. The results of five separate analyses of portions of the skin of a male Sprague Dawley rat showed a mean value of 84.5 +/- 4.1 (SEM) ng per g. Analyses of hair samples of ten male Sprague Dawley rats indicated a mean level of 143 +/- 19 (SEM) ng per g of hair. Most (approximately 72%) of the 15-ketosterol in hair was esterified. This report constitutes the first isolation of the 15-ketosterol from animal tissues. |
| | |
Authors:
|
G T Emmons; J St Pyrek; R Dam; M Martin; K Kudo; G J Schroepfer |
Related Documents
:
|
16175127 - Windows of detection of tetrazepam in urine, oral fluid, beard, and hair, with a specia... 16008057 - Automated multiple development thin-layer chromatography for separation of opiate alkal... 1501467 - Forensic drug testing for opiates. iv. analytical sensitivity, specificity, and accurac... 16261327 - Detection of testosterone, nandrolone and precursors in horse hair. 23008527 - Preparative isolation and purification of three glycine-conjugated cholic acids from pu... 7770517 - Cadmium-sensitive, cad1 mutants of arabidopsis thaliana are phytochelatin deficient. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of lipid research Volume: 29 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 1988 Aug |
Date Detail:
|
Created Date: 1988-12-16 Completed Date: 1988-12-16 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1039-54 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry, Rice University, Houston, TX 77251. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Anticholesteremic Agents / analysis* Cholestenes / analysis* Cholestenones / analysis* Cholesterol / metabolism* Esters / analysis Hair / analysis Male Rats Rats, Inbred Strains Skin / analysis* |
| Grant Support | |
ID/Acronym/Agency:
|
HL-15376/HL/NHLBI NIH HHS; HL-22532/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Anticholesteremic Agents; 0/Cholestenes; 0/Cholestenones; 0/Esters; 50673-97-7/cholest-8(14)-en-3-ol-15-one; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Assay of unesterified cholesterol-5,6-epoxide in human serum by isotope dilution mass spectrometry. ...
Next Document: Biliary proteins: assessment of quantitative techniques and comparison in gallstone and nongallstone...