Document Detail


5-Octadecenoic acid: evidence for a novel type of fatty acid modification in schistosomes.
MedLine Citation:
PMID:  9716488     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The lipid metabolism of schistosomes is characterized by several intriguing adaptations to a parasitic way of living. The surface of the parasite consists of two closely apposed phospholipid bilayers, a structure unique to blood flukes. Schistosomes do not synthesize fatty acids de novo, but are able to modify fatty acids, which they obtain from the host, by chain elongation. Here we present evidence that schistosomes are capable of another type of fatty acid modification, resulting in the formation of 5-octadecenoic acid [C18:1(5)]. This highly unusual fatty acid, which is absent in the blood of the host, was shown to be almost exclusively located in the outer membrane complex of the schistosome. Within these membranes, it was almost exclusively present in one molecular phospholipid species, 1-palmitoyl-2,5-octadecenoyl phosphatidylcholine [C16:0-18:1(5)PtdCho]. Apart from dipalmitoyl phosphatidylcholine, this was the most abundant phosphatidylcholine species in the outer membrane complex. The specific synthesis by the schistosome of C18:1(5) and the highly specific localization of this fatty acid to the tegumental membranes suggest an important tegument-mediated role for this lipid.
Authors:
J F Brouwers; C Versluis; L M van Golde; A G Tielens
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  334 ( Pt 2)     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1998-10-22     Completed Date:  1998-10-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  315-9     Citation Subset:  IM    
Affiliation:
Laboratory of Veterinary Biochemistry and Institute of Biomembranes, Utrecht University, P.O. Box 80176, 3508 TD Utrecht, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatography, High Pressure Liquid
Isomerism
Oleic Acid
Phospholipids / chemistry,  metabolism*
Schistosoma mansoni / chemistry,  metabolism*
Spectrometry, Mass, Secondary Ion
Stearic Acids / analysis,  metabolism*
Chemical
Reg. No./Substance:
0/5-octadecenoic acid; 0/Phospholipids; 0/Stearic Acids; 112-80-1/Oleic Acid
Comments/Corrections

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