Document Detail


The 5-O-beta-D-galactofuranosyl-containing glycopeptide from Penicillium charlesii. Identification of phosphocholine attached to C-6 of mannopyranosyl residues of the mannan region.
MedLine Citation:
PMID:  7061436     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Peptidophosphogalactomannan from Penicillium charlesii contains ethanolamine, presumably linked via phosphodiester to galactomannan (Rick, P. D., Drewes, L. R., and Gander, J. E., (1974) J. Biol. Chem. 249, 2072-2078). These investigations have been extended using 13C and 31P NMR spectroscopy to show that peptidophosphogalactomannan also contains choline and that both ethanolamine and choline are linked through phosphodiester linkages to C-8 of mannosyl residues of the mannan region of peptidophosphogalactomannan. Approximately one-half of the bases derived from ethanolamine, and which are attached to the mannan as phosphodiesters, are released as choline upon treatment with dilute alkali. It was shown that 13C from L-[methyl-13C]methionine is incorporated into methyl groups of mannosyl-6-phosphocholine in vivo. There were no 13C NMR signals from the methyl carbons of either 2-methylaminoethanol (33.3 ppm) or 2-dimethylaminoethanol (45.9 ppm) observed in these experiments even though the major signal was from the methyl carbons of choline (56.9 ppm).
Authors:
C J Unkefer; C Jackson; J E Gander
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  257     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1982 Mar 
Date Detail:
Created Date:  1982-05-21     Completed Date:  1982-05-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2491-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Choline / analysis
Ethanolamines / analysis
Galactosides / analysis*
Glycopeptides / analysis*
Glycosides / analysis*
Magnetic Resonance Spectroscopy
Penicillium / analysis*
Grant Support
ID/Acronym/Agency:
GM 19978/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Ethanolamines; 0/Galactosides; 0/Glycopeptides; 0/Glycosides; 62-49-7/Choline

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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