| The 5-O-beta-D-galactofuranosyl-containing glycopeptide from Penicillium charlesii. Identification of phosphocholine attached to C-6 of mannopyranosyl residues of the mannan region. | |
MedLine Citation:
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PMID: 7061436 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Peptidophosphogalactomannan from Penicillium charlesii contains ethanolamine, presumably linked via phosphodiester to galactomannan (Rick, P. D., Drewes, L. R., and Gander, J. E., (1974) J. Biol. Chem. 249, 2072-2078). These investigations have been extended using 13C and 31P NMR spectroscopy to show that peptidophosphogalactomannan also contains choline and that both ethanolamine and choline are linked through phosphodiester linkages to C-8 of mannosyl residues of the mannan region of peptidophosphogalactomannan. Approximately one-half of the bases derived from ethanolamine, and which are attached to the mannan as phosphodiesters, are released as choline upon treatment with dilute alkali. It was shown that 13C from L-[methyl-13C]methionine is incorporated into methyl groups of mannosyl-6-phosphocholine in vivo. There were no 13C NMR signals from the methyl carbons of either 2-methylaminoethanol (33.3 ppm) or 2-dimethylaminoethanol (45.9 ppm) observed in these experiments even though the major signal was from the methyl carbons of choline (56.9 ppm). |
Authors:
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C J Unkefer; C Jackson; J E Gander |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 257 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1982 Mar |
Date Detail:
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Created Date: 1982-05-21 Completed Date: 1982-05-21 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2491-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Choline
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analysis Ethanolamines / analysis Galactosides / analysis* Glycopeptides / analysis* Glycosides / analysis* Magnetic Resonance Spectroscopy Penicillium / analysis* |
| Grant Support | |
ID/Acronym/Agency:
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GM 19978/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Ethanolamines; 0/Galactosides; 0/Glycopeptides; 0/Glycosides; 62-49-7/Choline |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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