Document Detail

5'-Nucleotidase levels in normal and virus-transformed cells: implications for cellular aging in vitro.
MedLine Citation:
PMID:  449279     Owner:  NLM     Status:  MEDLINE    
5'-Nucleotidase activity in the postnuclear supernatant of normal and SV-40 virus-transformed human embryonic lung, chick embryo, and mouse embryo fibroblasts, WI-38, IMR-90, VA-13, CEF, 3T3, and SV3T3 cells was measured. In IMR-90 and CEF cells, both of which have a limited lifespan in vitro, 5'-nucleotidase activity increased 6- and 20-fold, respectively, with increasing population doublings. However, VA-13 cells, a permanent cell line, showed no increase in this enzyme activity with progressive population doublings. 5'-Nucleotidase activity was not detectable in two other permanent cell lines, 3T3 and SV3T3 cells. Of the six cell lines tested, the conspicuous enhancement in 5'-nucleotidase activity with increasing population doublings was observed only in normal cell lines and was absent in transformed cell lines. These observations suggest a molecular mechanism which may play a role in aging of normal cells in vitro and which may involve catabolism of nucleic acids.
A S Sun; L J Alvarez; P S Reinach; E Rubin
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Laboratory investigation; a journal of technical methods and pathology     Volume:  41     ISSN:  0023-6837     ISO Abbreviation:  Lab. Invest.     Publication Date:  1979 Jul 
Date Detail:
Created Date:  1979-09-01     Completed Date:  1979-09-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376617     Medline TA:  Lab Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1-4     Citation Subset:  IM    
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MeSH Terms
Adenosine Monophosphate / metabolism
Cell Line
Cell Survival*
Cell Transformation, Viral*
Chick Embryo
Fibroblasts / enzymology
Nucleotidases / metabolism*
Reg. No./Substance:
61-19-8/Adenosine Monophosphate; EC 3.1.3.-/Nucleotidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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