| 5'-Nucleotidase levels in normal and virus-transformed cells: implications for cellular aging in vitro. | |
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MedLine Citation:
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PMID: 449279 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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5'-Nucleotidase activity in the postnuclear supernatant of normal and SV-40 virus-transformed human embryonic lung, chick embryo, and mouse embryo fibroblasts, WI-38, IMR-90, VA-13, CEF, 3T3, and SV3T3 cells was measured. In IMR-90 and CEF cells, both of which have a limited lifespan in vitro, 5'-nucleotidase activity increased 6- and 20-fold, respectively, with increasing population doublings. However, VA-13 cells, a permanent cell line, showed no increase in this enzyme activity with progressive population doublings. 5'-Nucleotidase activity was not detectable in two other permanent cell lines, 3T3 and SV3T3 cells. Of the six cell lines tested, the conspicuous enhancement in 5'-nucleotidase activity with increasing population doublings was observed only in normal cell lines and was absent in transformed cell lines. These observations suggest a molecular mechanism which may play a role in aging of normal cells in vitro and which may involve catabolism of nucleic acids. |
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Authors:
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A S Sun; L J Alvarez; P S Reinach; E Rubin |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Laboratory investigation; a journal of technical methods and pathology Volume: 41 ISSN: 0023-6837 ISO Abbreviation: Lab. Invest. Publication Date: 1979 Jul |
Date Detail:
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Created Date: 1979-09-01 Completed Date: 1979-09-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376617 Medline TA: Lab Invest Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1-4 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Monophosphate
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metabolism Animals Cell Line Cell Survival* Cell Transformation, Viral* Chick Embryo Female Fibroblasts / enzymology Humans Mice Nucleotidases / metabolism* |
| Chemical | |
Reg. No./Substance:
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61-19-8/Adenosine Monophosphate; EC 3.1.3.-/Nucleotidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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