Document Detail

5'-Nucleotidase of L 1210 cells. Cytochemical and kinetic studies.
MedLine Citation:
PMID:  437576     Owner:  NLM     Status:  MEDLINE    
The activity of 5'-nucleotidase in L 1210 leukaemia cells fixed in situ by glutaraldehyde was established by electron microscopic cytochemistry and biochemical assays. The enzyme activity is localized uniquely on the cell surface. In biochemical assays the time course of phosphate liberation by the suspension of glutaraldehyde fixed cells was linear up to 15 min incubation. The activity was proportional to the number of cells in the suspension, amounting of 0.019 units activity per 10(8) cells.
D D Ban; J Kawiak
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Folia histochemica et cytochemica     Volume:  17     ISSN:  0015-5586     ISO Abbreviation:  Folia Histochem Cytochem (Krakow)     Publication Date:  1979  
Date Detail:
Created Date:  1979-07-25     Completed Date:  1979-07-25     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  2984760R     Medline TA:  Folia Histochem Cytochem (Krakow)     Country:  POLAND    
Other Details:
Languages:  eng     Pagination:  29-36     Citation Subset:  IM    
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MeSH Terms
Cell Membrane / enzymology,  ultrastructure
Leukemia L1210 / enzymology*,  ultrastructure
Nucleotidases / metabolism*
Reg. No./Substance:
EC 3.1.3.-/Nucleotidases

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