Document Detail


5-(N-Ethyl-N-isopropyl)-amiloride inhibits amino acid release from the ischemic rat cerebral cortex: role of Na+-H+ exchange.
MedLine Citation:
PMID:  9813378     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of the selective Na+/H+ antiporter inhibitor 5-(N-ethyl-N-isopropyl)-amiloride (EIPA) on amino acid release from the ischemic/reperfused rat cerebral cortex was investigated using a cortical cup technique. EIPA (25 microM in artificial cerebrospinal fluid), applied topically, inhibited the ischemia-reperfusion evoked efflux of aspartate, glutamate, gamma-aminobutyric acid, taurine and phosphoethanolamine. Reductions in the ischemia-evoked releases of these amino acids suggest that ischemia precipitates acidosis, Na+/H+ exchange and cell swelling with amino acid release as the cells mount a regulatory volume decrease response. EIPA, by blocking Na+/H+ exchange, would reduce cell swelling and the resulting amino acid release.
Authors:
J W Phillis; M H O'Regan; D Song
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Brain research     Volume:  812     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1999-02-10     Completed Date:  1999-02-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  297-300     Citation Subset:  IM    
Copyright Information:
Copyright 1998 Elsevier Science B.V.
Affiliation:
Department of Physiology, Wayne State University School of Medicine, 540 E. Canfield, Detroit, MI 48201, USA. jphillis@med.wayne.edu
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MeSH Terms
Descriptor/Qualifier:
Amiloride / analogs & derivatives*,  pharmacology
Amino Acids / metabolism*
Animals
Brain Ischemia / drug therapy*
Male
Rats
Rats, Sprague-Dawley
Sodium-Hydrogen Antiporter / antagonists & inhibitors*
Grant Support
ID/Acronym/Agency:
NS26912-09/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Sodium-Hydrogen Antiporter; 1154-25-2/ethylisopropylamiloride; 2609-46-3/Amiloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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