Document Detail

5-Hydroxytryptamine uptake in oxygen radical-mediated acute lung injury.
MedLine Citation:
PMID:  2913887     Owner:  NLM     Status:  MEDLINE    
Pulmonary endothelial cell function (ECF) studies have been shown to be a sensitive indicator of chronic lung injury. We attempted to correlate changes in 5-hydroxytryptamine (5HT) uptake with an acute oxygen radical-mediated lung injury in dogs. Beta-d glucose/glucose oxidase was injected intravenously in an experimental group (n = 10), while the control group (n = 5) received saline. 5HT uptake, measured using a multiple indicator dilution technique before and 20 min after injection, was calculated for both the percent total uptake and the peak extraction ratio of 5HT during a single passage through the lung. The mean pulmonary and systemic arterial pressures (PAP, SAP), total pulmonary resistance (TPR), extravascular lung water (EVLW), and wet-to-dry weight ratios were also determined. The experimental group showed an acute rise in PAP and TPR and a fall in SAP after the injection, all returning to normal by 20 min; total 5HT uptake fell from 81 +/- 2.3% to 47 +/- 6.5% (p = 0.0002) as did the peak extraction ratio from 0.87 +/- 0.013 to 0.44 +/- 0.066 (p = 0.0001). No change in 5HT uptake was observed in the control group. EVLW did not change in either group, but wet-to-dry weight ratio was elevated in the experimental group (5.21 +/- 0.12 versus 4.73 +/- 0.06, p less than 0.01). ECF studies of 5HT uptake appear to be a sensitive indicator of acute lung injury in this large-animal, oxygen radical-induced injury model.
W T Vigneswaran; H S Stanbrook; R Doctor; S McCormick; J Schwappach; M R Johnston
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The American review of respiratory disease     Volume:  139     ISSN:  0003-0805     ISO Abbreviation:  Am. Rev. Respir. Dis.     Publication Date:  1989 Feb 
Date Detail:
Created Date:  1989-03-09     Completed Date:  1989-03-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0370523     Medline TA:  Am Rev Respir Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  382-6     Citation Subset:  AIM; IM    
Department of Surgery, University of Colorado School of Medicine, Denver.
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MeSH Terms
Acute Disease
Carbon Radioisotopes / diagnostic use
Endothelium / drug effects,  metabolism
Extracellular Space / drug effects,  metabolism
Free Radicals
Glucose / pharmacology
Glucose Oxidase / pharmacology
Hemodynamics / drug effects
Lung / drug effects,  metabolism*
Lung Injury*
Organ Size / drug effects
Oxygen Consumption* / drug effects
Serotonin / metabolism*
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Free Radicals; 50-67-9/Serotonin; 50-99-7/Glucose; EC Oxidase

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