| 5-Fluorouracil induces structural changes in rat liver tRNA. | |
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MedLine Citation:
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PMID: 8081204 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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5-fluorouracil (FUra) has been shown to modulate the aminoacylation function of rat liver tRNA. The present study was aimed at studying the structure-function relationship of FUra-substituted tRNA. Male Wistar rats (2-3 month old) were given a single i.p. injection of FUra at 50, 250, or 500 mg/kg body wt. and FUra-substituted total liver tRNA, i.e. tRNA(FUra50, 250, and 500, respectively, were isolated 3 h later. Normal tRNA (tRNA(N)) was isolated from saline-treated control rats. Thermal denaturation studies showed higher melting temperatures for tRNA(FUra) compared to tRNA(N). Heat denaturation followed by renaturation of total tRNA did not affect the activity of tRNA(N) and tRNA(FUra50), where as tRNA(FUra250 and 500) lost 35% and 72% of activity, respectively, compared to the corresponding group of non-denatured tRNA. Antibodies specific to rat liver tRNA recognized normal and FUra-substituted tRNA in the order of tRNA(N) > tRNA(FUra50) > or = tRNA(FUra250) > tRNA(FUra500) in an avidin-biotin micro-enzyme linked immunosorbant assay. tRNA(N) or tRNA(FUra50) preincubated with tRNA antiserum showed 74% and 59% of aminoacylation activity, respectively, compared to that of corresponding tRNA preincubated with normal rabbit IgG. However, activities of similarly treated tRNA(FUra250 and 500) were not affected. The observations of possible changes in the secondary structure of rat liver tRNA upon incorporation of FUra are discussed. |
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Authors:
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S V Vulimiri; R Nayak |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemistry and molecular biology international Volume: 33 ISSN: 1039-9712 ISO Abbreviation: Biochem. Mol. Biol. Int. Publication Date: 1994 May |
Date Detail:
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Created Date: 1994-10-11 Completed Date: 1994-10-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9306673 Medline TA: Biochem Mol Biol Int Country: AUSTRALIA |
Other Details:
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Languages: eng Pagination: 147-54 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acylation
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drug effects Animals Fluorouracil / pharmacology* Liver / drug effects* Male Nucleic Acid Conformation / drug effects Nucleic Acid Denaturation RNA, Transfer, Amino Acyl / drug effects* Rats Rats, Wistar Temperature |
| Chemical | |
Reg. No./Substance:
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0/RNA, Transfer, Amino Acyl; 51-21-8/Fluorouracil |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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