| 5-Bromodeoxyuridine induces senescence-like phenomena in mammalian cells regardless of cell type or species. | |
| | |
MedLine Citation:
|
PMID: 10578056 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
5-Bromodeoxyuridine was found to induce flat and enlarged cell shape, characteristics of senescent cells, and senescence-associated beta-galactosidase in mammalian cells regardless of cell type or species. In immortal human cells, fibronectin, collagenase I, and p21(wafl/sdi-1) mRNAs were immediately and very strongly induced, and the mortality marker mortalin changed to the mortal type from the immortal type. Human cell lines lacking functional p21(wafl/sdi-1), p16(ink4a), or p53 behaved similarly. The protein levels of p16(ink4a) and p53 did not change uniformly, while the level of p21(wafl/sdi-1) was increased by varying degrees in positive cell lines. Telomerase activity was suppressed in positive cell lines, but accelerated telomere shortening was not observed in tumor cell lines. These results suggest that 5-bromodeoxyuridine activates a common senescence pathway present in both mortal and immortal mammalian cells. |
| | |
Authors:
|
E Michishita; K Nakabayashi; T Suzuki; S C Kaul; H Ogino; M Fujii; Y Mitsui; D Ayusawa |
Related Documents
:
|
10617776 - The mrna phenotype of a human rpe cell line at replicative senescence. 16668076 - Ethylene production by growing and senescing pear fruit cell suspensions in response to... 17686666 - Changes in the level and distribution of ku proteins during cellular senescence. 20109216 - Senescent vs. non-senescent cells in the human annulus in vivo: cell harvest with laser... 18499746 - Sexual differentiation of vasopressin innervation of the brain: cell death versus pheno... 20883816 - Iqgap1 translocates to the nucleus in early s-phase and contributes to cell cycle progr... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of biochemistry Volume: 126 ISSN: 0021-924X ISO Abbreviation: J. Biochem. Publication Date: 1999 Dec |
Date Detail:
|
Created Date: 2000-03-28 Completed Date: 2000-03-28 Revised Date: 2007-12-19 |
Medline Journal Info:
|
Nlm Unique ID: 0376600 Medline TA: J Biochem Country: JAPAN |
Other Details:
|
Languages: eng Pagination: 1052-9 Citation Subset: IM |
Affiliation:
|
Kihara Institute for Biological Research and Graduate School of Integrated Science, Yokohama City University, Totsuka-ku, Yokohama, 244-0813, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aging
/
drug effects* Animals Biological Markers Bromodeoxyuridine / pharmacology* Cell Cycle Cell Line Electrophoresis, Polyacrylamide Gel Humans Mice Polymorphism, Restriction Fragment Length Telomere Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
|
0/Biological Markers; 59-14-3/Bromodeoxyuridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Molecular cloning of cDNA encoding a novel microphthalmia-associated transcription factor isoform wi...
Next Document: The effect of phospholipase A(2) immobilization upon calcium interaction: a kinetic study.